Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro
Terpenoids constitute a large and diverse class of natural products that serve many
functions in nature. Most of the tens of thousands of the discovered terpenoids are …
functions in nature. Most of the tens of thousands of the discovered terpenoids are …
Efflux pumps and antimicrobial resistance: Paradoxical components in systems genomics
Efflux pumps play a major role in the increasing antimicrobial resistance rendering a large
number of drugs of no use. Large numbers of pathogens are becoming multidrug resistant …
number of drugs of no use. Large numbers of pathogens are becoming multidrug resistant …
M utagenic O rganized R ecombination P rocess by H omologous In Vivo G rouping (MORPHING) for Directed Enzyme Evolution
D Gonzalez-Perez, P Molina-Espeja, E Garcia-Ruiz… - PLoS …, 2014 - journals.plos.org
Approaches that depend on directed evolution require reliable methods to generate DNA
diversity so that mutant libraries can focus on specific target regions. We took advantage of …
diversity so that mutant libraries can focus on specific target regions. We took advantage of …
Effects of chromosomal gene copy number and locations on polyhydroxyalkanoate synthesis by Escherichia coli and Halomonas sp.
Chromosomal integration and expression of heterologous gene (s) are favored in industrial
biotechnology due to the inheriting expression stability. Yet, chromosomal expression is …
biotechnology due to the inheriting expression stability. Yet, chromosomal expression is …
Yeast homologous recombination cloning leading to the novel peptides ambactin and xenolindicin
O Schimming, F Fleischhacker, FI Nollmann… - …, 2014 - Wiley Online Library
Heterologous production of GameXPeptide A (1), as well as of the novel peptide natural
products ambactin (2) and xenolindicins A–C (3 a–c), was achieved by using the “overlap …
products ambactin (2) and xenolindicins A–C (3 a–c), was achieved by using the “overlap …
Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp.
Since halophile Halomonas spp. can grow contamination free in seawater under unsterile
and continuous conditions, it holds great promise for industrial biotechnology to produce low …
and continuous conditions, it holds great promise for industrial biotechnology to produce low …
LncRNA RUS shapes the gene expression program towards neurogenesis
MF Schneider, V Müller, SA Müller… - Life science …, 2022 - life-science-alliance.org
The evolution of brain complexity correlates with an increased expression of long,
noncoding (lnc) RNAs in neural tissues. Although prominent examples illustrate the potential …
noncoding (lnc) RNAs in neural tissues. Although prominent examples illustrate the potential …
Computer-aided design for metabolic engineering
The development and application of biotechnology-based strategies has had a great socio-
economical impact and is likely to play a crucial role in the foundation of more sustainable …
economical impact and is likely to play a crucial role in the foundation of more sustainable …
A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli
Background The chromosomal integration of biological parts in the host genome enables
the engineering of plasmid-free stable strains with single-copy insertions of the desired gene …
the engineering of plasmid-free stable strains with single-copy insertions of the desired gene …
A variety of simple and ultra-low-cost methods preparing SLiCE extracts and their application to DNA cloning
R Guo, W Zhao, L Wei, S Zhang, L Feng… - Journal of Microbiological …, 2022 - Elsevier
Cell lysates from a laboratory strain of Escherichia coli can be exploited for seamless DNA
cloning in vitro, which is named the seamless ligation cloning extract (SLiCE) cloning …
cloning in vitro, which is named the seamless ligation cloning extract (SLiCE) cloning …