Transgenesis procedures in Xenopus

A Chesneau, LM Sachs, N Chai, Y Chen… - Biology of the …, 2008 - Wiley Online Library
Stable integration of foreign DNA into the frog genome has been the purpose of several
studies aimed at generating transgenic animals or producing mutations of endogenous …

Chromosome banding in Amphibia. XXXII. The genus Xenopus (Anura, Pipidae)

M Schmid, C Steinlein - Cytogenetic and genome research, 2015 - karger.com
Mitotic chromosomes of 16 species of the frog genus Xenopus were prepared from kidney
and lung cell cultures. In the chromosomes of 7 species, high-resolution replication banding …

Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog (Rana rugosa, Ranidae …

Y Uno, C Nishida, Y Oshima, S Yokoyama, I Miura… - Chromosome …, 2008 - Springer
There are regional variations of sex chromosome morphologies in the Japanese wrinkled
frog, Rana rugosa (2n= 26): heterogametic ZZ/ZW-type and XX/XY-type sex chromosomes …

Mapping of single-copy genes by TSA-FISH in the codling moth, Cydia pomonella

LZ Carabajal Paladino, P Nguyen, J Šíchová, F Marec - BMC genetics, 2014 - Springer
Background We work on the development of transgenic sexing strains in the codling moth,
Cydia pomonella (Tortricidae), which would enable to produce male-only progeny for the …

Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH

V Krylov, S Kubickova, J Rubes, J Macha… - Chromosome …, 2010 - Springer
Laser microdissection was used for the preparation of whole chromosome painting probes
in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ …

Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA

V Krylov, T Tlapakova, J Macha - Cytogenetic and Genome Research, 2007 - karger.com
A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on
Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide …

Insights from Xenopus genomes

N Pollet, A Mazabraud - Vertebrate Genomes, 2006 - karger.com
Amphibians have been used since the 19th century as vertebrate models for the
experimentalist. Since 50 years or so, Xenopus laevis is the most widely used anuran …

Xenopus cytogenetics and chromosomal evolution

V Krylov, T Tlapakova - Cytogenetic and Genome Research, 2015 - karger.com
The genus Xenopus represents important model organisms in the field of developmental
biology and chromosomal evolution. Developmental processes are tightly coupled with the …

Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes

T Tlapakova, V Krylov, J Macha - Chromosome Research, 2005 - Springer
Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis
have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ …

[PDF][PDF] Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next …

V Krylov, T Tlapakova, J Macha, J Curlej… - Folia …, 2008 - nempov.lf1.cuni.cz
For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of
transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted …