Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Droplet-based microfluidics in biomedical applications

L Amirifar, M Besanjideh, R Nasiri, A Shamloo… - …, 2022 - iopscience.iop.org
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Droplet microfluidics: A tool for biology, chemistry and nanotechnology

S Mashaghi, A Abbaspourrad, DA Weitz… - TrAC Trends in …, 2016 - Elsevier
The ability to perform laboratory operations on small scales using miniaturized devices
provides numerous benefits, including reduced quantities of reagents and waste as well as …

Controlled droplet microfluidic systems for multistep chemical and biological assays

TS Kaminski, P Garstecki - Chemical Society Reviews, 2017 - pubs.rsc.org
Droplet microfluidics is a relatively new and rapidly evolving field of science focused on
studying the hydrodynamics and properties of biphasic flows at the microscale, and on the …

New and developing diagnostic technologies for urinary tract infections

M Davenport, KE Mach, LMD Shortliffe… - Nature Reviews …, 2017 - nature.com
Timely and accurate identification and determination of the antimicrobial susceptibility of
uropathogens is central to the management of UTIs. Urine dipsticks are fast and amenable …

Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes

WJ Watterson, M Tanyeri, AR Watson, CM Cham… - Elife, 2020 - elifesciences.org
Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and
yield biased sampling of environmental microbes due to ecological and evolutionary factors …

Concentration gradient generation methods based on microfluidic systems

X Wang, Z Liu, Y Pang - RSC advances, 2017 - pubs.rsc.org
Various concentration gradient generation methods based on microfluidic systems are
summarized in this paper. The review covers typical structural characteristics, gradient …

Selection for antimicrobial resistance is reduced when embedded in a natural microbial community

U Klümper, M Recker, L Zhang, X Yin, T Zhang… - The ISME …, 2019 - academic.oup.com
Antibiotic resistance has emerged as one of the most pressing, global threats to public
health. In single-species experiments selection for antibiotic resistance occurs at very low …