Gene therapies are currently one of the most investigated therapeutic modalities in both the
preclinical and clinical settings and have shown promise in treating a diverse spectrum of …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

The three-dimensional positions of atoms in protein molecules define their structure and
their roles in biological processes. The more precisely atomic coordinates are determined …

Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Cryo-electron microscopy (cryo-EM) has emerged as an unprecedented tool to resolve
protein structures at atomic resolution. Structural insights of biological samples not …

Determining high-resolution structures of biological macromolecules amassing less than
100 kilodaltons (kDa) has been a longstanding goal of the cryo-electron microscopy (cryo …

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it
more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular …

Parvoviruses, infecting vertebrates and invertebrates, are a family of single-stranded DNA
viruses with small, non-enveloped capsids with T= 1 icosahedral symmetry. A quarter of a …

While the application of cryogenic electron microscopy (cryo-EM) to helical polymers in
biology has a long history, due to the huge number of helical macromolecular assemblies in …

Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle
movement during imaging, which remains poorly understood. We show that this movement …