There is no doubt that the existing arsenal of antimicrobial agents we have in hand for the
treatment of infectious diseases is insufficient to protect us over the long term. 1-3 The …

This review focuses on target-based approaches for developing new chemical classes of
antibacterial agents aimed at the bacterial cell wall. The clinical success of antibiotics such …

Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential
genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 …

To address the need for new approaches to antibiotic drug development, we have identified
a large number of essential genes for the bacterial pathogen, Staphylococcus aureus, using …

Comprehensive genomic analysis of the important human pathogen Staphylococcus aureus
was achieved by a strategy involving antisense technology in a regulatable gene expression …

Neisseria gonorrhoeae homologues of gyrA and parC have been identified using
hybridization probes generated from conserved regions of diverse gyrA genes. These genes …

Identification of genes that encode essential products provides a promising approach to
validation of new antibacterial drug targets. We have developed a mariner-based …

An increasing number of microbial genomes have been completely sequenced, and
functional analyses of these genomic sequences are under way. To facilitate these …

The availability of genome sequences is revolutionizing the field of microbiology. Genetic
methods are being modified to facilitate rapid analysis at a genome-wide level and are …

Successful small-molecule antibacterial agents must meet a variety of criteria. Foremost is
the need for selectivity and safety: It is easy to kill bacteria with chemicals, but difficult to do it …