Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein
profiling methods as a major pillar for cellular atlas building of complex samples, from …

Unravelling spatio-temporal patterns of gene expression is crucial to understanding core
biological principles from embryogenesis to disease. Here we review emerging …

INTRODUCTION Microbial populations display heterogeneous gene expression profiles that
result in phenotypic differences between individual bacteria. This diversity can allow …

Visualization of the transcriptome and the nuclear organization in situ has been challenging
for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method …

INTRODUCTION Cell differentiation is classically described as a unidirectional process that
progresses through a series of lineage restriction events, with cellular potential being …

The ability of the pluripotent epiblast to contribute progeny to all three germ layers is thought
to be lost after gastrulation. The later-forming neural crest (NC) rises from ectoderm and it …

The teeth are vertebrate-specific, highly specialized organs performing fundamental
functions of mastication and speech, the maintenance of which is crucial for orofacial …

Background Neuroblastoma is the most common solid tumor in infants accounting for
approximately 15% of all cancer-related deaths. Over 50% of high-risk neuroblastoma …

Multiplexed fluorescence in situ hybridization techniques have enabled cell-type
identification, linking transcriptional heterogeneity with spatial heterogeneity of cells …

In the neural primordium of vertebrate embryos, the neural crest (NC) displays a unique
character: the capacity of its component cells to leave the neural primordium, migrate along …