Best practices for collecting and preserving marine mammal biological samples in the 'omics era

AM Van Cise, AD Switzer, A Apprill… - Marine Mammal …, 2024 - Wiley Online Library
The recent rise of 'omics and other molecular research technologies alongside improved
techniques for tissue preservation have broadened the scope of marine mammal research …

iTRAQ-based quantitative proteomic comparison of 2D and 3D adipocyte cell models co-cultured with macrophages using online 2D-nanoLC-ESI-MS/MS

SY Lee, SB Park, YE Kim, HM Yoo, J Hong, KJ Choi… - Scientific Reports, 2019 - nature.com
The demand for novel three-dimensional (3D) cell culture models of adipose tissue has
been increasing, and proteomic investigations are important for determining the underlying …

[HTML][HTML] Generational Diet-Induced Obesity Remodels the Omental Adipose Proteome in Female Mice

N Schuster-Little, M McCabe, K Nenninger… - Nutrients, 2024 - mdpi.com
Obesity, a complex condition that involves genetic, environmental, and behavioral factors, is
a non-infectious pandemic that affects over 650 million adults worldwide with a rapidly …

Ex vivo and in vitro methods as a platform for studying anthropogenic effects on marine mammals: four challenges and how to meet them

JM Vazquez, JI Khudyakov, CB Madelaire… - Frontiers in Marine …, 2024 - frontiersin.org
Marine mammals are integral to global biodiversity and marine health through their roles in
coastal, benthic, and pelagic ecosystems. Marine mammals face escalating threats from …

The plasma proteome reveals markers of recent and repeated stress in free-ranging seals

JG Avalos, CD Champagne, DE Crocker… - Conservation …, 2024 - academic.oup.com
Animals in nature potentially experience multiple stressors, and those of anthropogenic
origin are likely to be repeated or chronic. However, stress hormone levels are highly …

Proteomic investigation of a diseased gorgonian coral indicates disruption of essential cell function and investment in inflammatory and other immune processes

CA Ricci, AHM Kamal, JK Chakrabarty… - Integrative and …, 2019 - academic.oup.com
As scleractinian coral cover declines in the face of increased frequency in disease
outbreaks, future reefs may become dominated by octocorals. Understanding octocoral …

Role of BGS13 in the Secretory Mechanism of Pichia pastoris

CA Naranjo, AD Jivan, MN Vo… - Applied and …, 2019 - Am Soc Microbiol
The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein
expression for over 30 years. Because P. pastoris secretes few of its own proteins, the …

Blubber proteome response to repeated ACTH administration in a wild marine mammal

J Deyarmin, R Hekman, C Champagne… - … and Physiology Part D …, 2020 - Elsevier
While the response to acute stress is adaptive in nature, repeated or chronic stress can
impact an animal's fitness by depleting its energy stores and suppressing immune function …

Process and workflow for preparation of disparate mouse tissues for proteomic analysis

SA Michaud, H Pětrošová, AM Jackson… - Journal of Proteome …, 2020 - ACS Publications
We investigated the effect of homogenization strategy and protein precipitation on
downstream protein quantitation using multiple reaction monitoring mass spectrometry …

Liver proteome response to torpor in a basoendothermic mammal, Tenrec ecaudatus, provides insights into the evolution of homeothermy

JI Khudyakov, MD Treat… - American Journal …, 2021 - journals.physiology.org
Many mammals use adaptive heterothermy (eg, torpor, hibernation) to reduce metabolic
demands of maintaining high body temperature (T b). Torpor is typically characterized by …