Nuclear DNA amounts in angiosperms: targets, trends and tomorrow

MD Bennett, IJ Leitch - Annals of botany, 2011 - academic.oup.com
Abstract Background and Aims The amount of DNA in an unreplicated gametic chromosome
complement is known as the C-value and is a key biodiversity character of fundamental …

Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants

EM Temsch, P Koutecký, T Urfus, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
The estimation of nuclear DNA content has been by far the most popular application of flow
cytometry in plants. Because flow cytometry measures relative fluorescence intensities of …

Estimation of nuclear DNA content in plants using flow cytometry

J Doležel, J Greilhuber, J Suda - Nature protocols, 2007 - nature.com
Flow cytometry (FCM) using DNA-selective fluorochromes is now the prevailing method for
the measurement of nuclear DNA content in plants. Ease of sample preparation and high …

Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species

J Loureiro, E Rodriguez, J Doležel, C Santos - Annals of botany, 2007 - academic.oup.com
Abstract Background and Aims After the initial boom in the application of flow cytometry in
plant sciences in the late 1980s and early 1990s, which was accompanied by development …

流式细胞术在植物学研究中的应用——检测植物核DNA 含量和倍性水平

田新民, 周香艳, 弓娜 - 2011 - ir.lzu.edu.cn
摘要流式细胞仪作为高效的检测工具, 在植物学研究的多个领域都发挥了重要作用.
兰州大学干旱与草地生态教育部重点实验室分子生态所通过大量的植物流式细胞术实验 …

The application of flow cytometry for estimating genome size, ploidy level endopolyploidy, and reproductive modes in plants

J Pellicer, RF Powell, IJ Leitch - Molecular plant taxonomy: methods and …, 2021 - Springer
Over the years, the amount of DNA in a nucleus (genome size) has been estimated using a
variety of methods, but increasingly, flow cytometry (FCM) has become the method of choice …

Application‐based guidelines for best practices in plant flow cytometry

E Sliwinska, J Loureiro, IJ Leitch, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
Flow cytometry (FCM) is currently the most widely‐used method to establish nuclear DNA
content in plants. Since simple, 1‐3‐parameter, flow cytometers, which are sufficient for most …

Applications of flow cytometry to evolutionary and population biology

P Kron, J Suda, BC Husband - Annu. Rev. Ecol. Evol. Syst., 2007 - annualreviews.org
Flow cytometry, a method of rapidly characterizing optical properties of cells and cell
components within individuals, populations, and communities, is advancing research in …

Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant

CR Carvalho, WR Clarindo, MM Praça, FS Araujo… - Plant Science, 2008 - Elsevier
In this report, we present the genome size, the base composition and the karyotype of
Jatropha curcas L., which is becoming an important oleaginous crop in tropical areas for …

Comparison of four nuclear isolation buffers for plant DNA flow cytometry

JÃO Loureiro, E Rodriguez, J DOLEŽEL… - Annals of …, 2006 - academic.oup.com
Background and Aims DNA flow cytometry requires preparation of suspensions of intact
nuclei, which are stained using a DNA-specific fluorochrome prior to analysis. Various buffer …