Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Biobased organic acids production by metabolically engineered microorganisms
Highlights•The use of inexpensive carbon sources is urgently needed for citric acid
production.•Low-pH process benefits lactic acid production.•Different strains and processes …
production.•Low-pH process benefits lactic acid production.•Different strains and processes …
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences
A Espah Borujeni, D Cetnar, I Farasat… - Nucleic acids …, 2017 - academic.oup.com
A mRNA's translation rate is controlled by several sequence determinants, including the
presence of RNA structures within the N-terminal regions of its coding sequences. However …
presence of RNA structures within the N-terminal regions of its coding sequences. However …
[HTML][HTML] A CRISPR-Cas9 assisted non-homologous end-joining strategy for one-step engineering of bacterial genome
T Su, F Liu, P Gu, H Jin, Y Chang, Q Wang, Q Liang… - Scientific reports, 2016 - nature.com
Homologous recombination-mediated genome engineering has been broadly applied in
prokaryotes with high efficiency and accuracy. However, this method is limited in realizing …
prokaryotes with high efficiency and accuracy. However, this method is limited in realizing …
White biotechnology: state of the art strategies for the development of biocatalysts for biorefining
S Heux, I Meynial-Salles, MJ O'Donohue… - Biotechnology …, 2015 - Elsevier
White biotechnology is a term that is now often used to describe the implementation of
biotechnology in the industrial sphere. Biocatalysts (enzymes and microorganisms) are the …
biotechnology in the industrial sphere. Biocatalysts (enzymes and microorganisms) are the …
Combinatorial pathway optimization for streamlined metabolic engineering
Highlights•Combinatorial pathway optimization for microbial production is highlighted.•
Frequently applied methods for diversification are critically scrutinized.•Important heuristics …
Frequently applied methods for diversification are critically scrutinized.•Important heuristics …
Recent insights into the genotype–phenotype relationship from massively parallel genetic assays
With the molecular revolution in Biology, a mechanistic understanding of the genotype–
phenotype relationship became possible. Recently, advances in DNA synthesis and …
phenotype relationship became possible. Recently, advances in DNA synthesis and …
Genome scale engineering techniques for metabolic engineering
Metabolic engineering has expanded from a focus on designs requiring a small number of
genetic modifications to increasingly complex designs driven by advances in genome-scale …
genetic modifications to increasingly complex designs driven by advances in genome-scale …
Unlocking nature's biosynthetic potential by directed genome evolution
Highlights•Directed genome evolution is a powerful tool to unlock microbial biosynthetic
potential.•Adaptive laboratory evolution is a traditional but efficient tool to improve cell …
potential.•Adaptive laboratory evolution is a traditional but efficient tool to improve cell …