Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Creating memories: molecular mechanisms of CRISPR adaptation
H Lee, DG Sashital - Trends in biochemical sciences, 2022 - cell.com
Prokaryotes use clustered regularly interspaced short palindromic repeat (CRISPR) and
CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems …
CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems …
Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays
A Santiago-Frangos, WS Henriques… - Nature Structural & …, 2023 - nature.com
Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of
foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific …
foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific …
Host nucleases generate prespacers for primed adaptation in the E. coli type IE CRISPR-Cas system
AA Shiriaeva, K Kuznedelov, I Fedorov… - Science …, 2022 - science.org
CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic
acids. In Escherichia coli, immunity is acquired upon integration of 33-bp spacers into …
acids. In Escherichia coli, immunity is acquired upon integration of 33-bp spacers into …
Cas1–Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation
T Killelea, JU Dimude, L He, AL Stewart… - Nucleic acids …, 2023 - academic.oup.com
Abstract Prokaryotic Cas1–Cas2 protein complexes generate adaptive immunity to mobile
genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De …
genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De …
CRISPR-Cas adaptation in Escherichia coli
Prokaryotes use the adaptive immunity mediated via the Clustered Regularly Interspaced
Short Palindromic Repeats and CRISPR associated (CRISPR-Cas) system for protection …
Short Palindromic Repeats and CRISPR associated (CRISPR-Cas) system for protection …
Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs
Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of
foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific …
foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific …
[HTML][HTML] Digging into the lesser-known aspects of CRISPR biology
NM Guzmán, B Esquerra-Ruvira, FJM Mojica - International Microbiology, 2021 - Springer
A long time has passed since regularly interspaced DNA repeats were discovered in
prokaryotes. Today, those enigmatic repetitive elements termed clustered regularly …
prokaryotes. Today, those enigmatic repetitive elements termed clustered regularly …
Assembly of Cas7 subunits of Leptospira on the mature crRNA of CRISPR-Cas IB is modulated by divalent ions
MS Hussain, M Kumar - Gene, 2022 - Elsevier
The spirochete Leptospira interrogans serovar Copenhageni harbors the genetic elements
of the CRISPR-Cas type IB system in its genome. CRISPR-Cas is a CRISPR RNA (crRNA) …
of the CRISPR-Cas type IB system in its genome. CRISPR-Cas is a CRISPR RNA (crRNA) …
Analysis of the features of 105 confirmed CRISPR loci in 487 Klebsiella variicola
Y Xi, J Zhao, J Zhang, Y Jin, H Yang… - Letters in Applied …, 2023 - academic.oup.com
Klebsiella variicola, an emerging human pathogen, poses a threat to public health. The
horizontal gene transfer (HGT) of plasmids is an important driver of the emergence of …
horizontal gene transfer (HGT) of plasmids is an important driver of the emergence of …