The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …

The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent
reporter molecules to monitor the production of amplification products during each cycle of …

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a
global health emergency, and its gene mutation and evolution further posed uncertainty of …

Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the
evident requirement for quantitative data analysis in molecular medicine, biotechnology …

Reverse transcription followed by quantitative polymerase chain reaction analysis, or qRT-
PCR, is an extremely sensitive, cost-effective method for quantifying gene transcripts from …

qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection
and quantification of RNA targets and is being utilized increasingly in novel clinical …

MicroRNAs are a class of small non-coding RNAs that serve as important regulators of gene
expression at the posttranscriptional level. They are stable in body fluids and pose great …

Lack of an objective, accurate, and noninvasive embryo assessment strategy remains one of
the major challenges encountered in in vitro fertilization. Cumulus and mural granulosa cells …