Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression,
production processes have steadily improved through numerous advances. In this review …

The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …

High cell density perfusion process of antibody producing CHO cells was developed in
disposable WAVE Bioreactor™ using external hollow fiber filter as cell separation device …

High cell densities in animal cell culture can be obtained by continuous perfusion of fresh
culture medium across hollow fiber membranes that retain the cells. Careful selection of the …

Current CHO cell production processes require an optimized space-time-yield. Process
intensification can support achieving this by enhancing the productivity and improving facility …

Continuous processes such as perfusion processes can offer advantages compared to fed‐
batch or batch processes in bio‐processing: improved product quality (eg for labile …

The interest for perfusion is increasing nowadays. This new focus has emerged from a
synergy of a demand for disposable equipment and the availability of robust cell separation …

During the past 10 years, single-use bioreactors have been well accepted in modern
biopharmaceutical production processes targeting high-value products. Up to now, such …

Continuous upstream processing in mammalian cell culture for recombinant protein
production holds promise to increase product yield and quality. To facilitate the design and …

Monoclonal antibodies (mAbs) are of great interest to the biopharmaceutical industry due to
their widely used application as human therapeutic and diagnostic agents. As such, mAb …