Mass spectrometry (MS) is a powerful analytical technique that typically involves sample
preparation and online analytical separation before MS detection. Traditional methods often …

In native mass spectrometry (MS) salts are indispensable for preserving the native structures
of biomolecules, but detrimental to mass sensitivity, resolution, and accuracy. Such a conflict …

Direct analysis of micro-biopsy samples by mass spectrometry at single-cell level still faces
major challenges. In this work, we developed a polarity gradient focusing dip-and-go …

Mass spectrometers are sensitive tools used to identify and quantify both small and large
analytes using the mass-to-charge ratios (m/z) of ions generated by electrospray ionization …

CBT-Cys click condensation reaction has a high second-order reaction rate constant and
has found wide applicability in recent years. However, its reaction mechanism has not been …

Comprehensive protein identification and concomitant structural probing of proteins are of
great biological significance. However, this is challenging to accomplish simultaneously in …

In situ living cell protein analysis would enable the structural identification and functional
interrogation of intracellular proteins in native cellular environments. Previously, we have …

Rationale As a powerful ambient ion source, atmospheric pressure (AP) matrix‐assisted
laser desorption/ionization (MALDI) enables direct analysis at atmospheric …

The identification of endogenous proteins as well as their binding to metal ions in living cells
is determined by combining pulsed electrophoretic separations with nanoelectrospray …

Salinity tolerance of ambient electric arc ionization (AEAI) was evaluated by comparing
electrospray ionization for various samples at NaCl concentrations from 0 to 1000 mmol/L …