Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

Organoids are in vitro miniaturized and simplified model systems of organs that have gained
enormous interest for modelling tissue development and disease, and for personalized …

Adult-stem-cell-derived organoids model human epithelial tissues ex vivo, which enables
the study of host–microbe interactions with great experimental control. This protocol …

Tissue and organ biology are very challenging to study in mammals, and progress can be
hindered, particularly in humans, by sample accessibility and ethical concerns. However …

Human intestinal morphogenesis establishes 3D epithelial microarchitecture and spatially
organized crypt–villus characteristics. This unique structure is necessary to maintain …

Constant remodeling of tight junctions to regulate trans-epithelial permeability is essential in
maintaining intestinal barrier functions and thus preventing diffusion of small molecules and …

The derivation of induced pluripotent stem cells (iPSCs) over a decade ago sparked
widespread enthusiasm for the development of new models of human disease, enhanced …

Trillions of microbes have evolved with and continue to live on and within human beings. A
variety of environmental factors can affect intestinal microbial imbalance, which has a close …

Organoids are 3D in vitro culture systems derived from self-organizing stem cells. They can
recapitulate the in vivo architecture, functionality, and genetic signature of original tissues …

The in vitro organoid model is a major technological breakthrough that has already been
established as an essential tool in many basic biology and clinical applications. This near …