Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review

J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …

Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects

M Shakeel, A Rodriguez, UB Tahir, F Jin - Biotechnology letters, 2018 - Springer
Whenever gene expression is being examined, it is essential that a normalization process is
carried out to eliminate non-biological variations. The use of reference genes, such as …

Identification and Validation of Reference Genes for the Normalization of Gene Expression Data in qRT-PCR Analysis in Aphis gossypii (Hemiptera: Aphididae)

KS Ma, F Li, PZ Liang, XW Chen, Y Liu… - Journal of Insect …, 2016 - academic.oup.com
To obtain accurate and reliable results from quantitative real-time reverse transcription
polymerase chain reaction (qRT-PCR) analysis, it is necessary to select suitable reference …

Nuclear receptor HR3 controls locust molt by regulating chitin synthesis and degradation genes of Locusta migratoria

X Zhao, Z Qin, W Liu, X Liu, B Moussian, E Ma… - Insect Biochemistry and …, 2018 - Elsevier
During growth and development of insects, the steroid hormone 20-Hydroxyecdysone (20E)
regulates the molting process through activation of a series of genes including E74, E75 and …

Selection and Evaluation of Reference Genes for Expression Analysis Using qRT-PCR in Chilo suppressalis (Lepidoptera: Pyralidae)

J Xu, MX Lu, YD Cui, YZ Du - Journal of Economic Entomology, 2017 - academic.oup.com
Quantitative real-time polymerase chain reaction (qRT-PCR) is a valuable tool for estimating
gene expression; however, the validity is largely dependent on the selection of stable …

LmCYP4G102: An oenocyte-specific cytochrome P450 gene required for cuticular waterproofing in the migratory locust, Locusta migratoria

Z Yu, X Zhang, Y Wang, B Moussian, KY Zhu, S Li… - Scientific Reports, 2016 - nature.com
Cytochrome P450 superfamily proteins play important roles in detoxification of xenobiotics
and during physiological and developmental processes. To contribute to our understanding …

Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

CH Shen, LJ Peng, YX Zhang, HR Zeng, HF Yu, L Jin… - Insects, 2022 - mdpi.com
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is
a momentous tool for calculating the expression levels of targeted genes across various …

Transcriptome-wide survey, gene expression profiling and exogenous chemical-induced transcriptional responses of cytochrome P450 superfamily genes in migratory …

X Zhang, X Kang, H Wu, K Silver, J Zhang, E Ma… - Insect biochemistry and …, 2018 - Elsevier
Cytochrome P450 monooxygenases (CYPs) belong to a large superfamily of heme-
containing enzymes catalyzing at least 60 different types of chemically distinct reactions …

Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression in Goji Fruit Fly (Neoceratitis asiatica Becker) under Developmental Stages …

H Wei, H Qiao, S Liu, X Yuan, C Xu - International Journal of Molecular …, 2022 - mdpi.com
Goji fruit fly, Neoceratitis asiatica, is a major pest on the well-known medicinal plant Lycium
barbarum. Dissecting molecular mechanisms of infestation and host selection of N. asiatica …

Lipophorin receptor is required for the accumulations of cuticular hydrocarbons and ovarian neutral lipids in Locusta migratoria

Y Zhao, W Liu, X Zhao, Z Yu, H Guo, Y Yang… - International Journal of …, 2023 - Elsevier
Lipophorin is the most abundant lipoprotein particle in insect hemolymph. Lipophorin
receptor (LPR) is a glycoprotein that binds to the lipophorin and mediates cellular uptake …