CRISPR Cpf1 proteins: structure, function and implications for genome editing
F Safari, K Zare, M Negahdaripour… - Cell & bioscience, 2019 - Springer
CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive
immune system, allows biologists to edit genomic DNA in a precise and specific way …
immune system, allows biologists to edit genomic DNA in a precise and specific way …
Applications of the CRISPR/Cas9 system for rice grain quality improvement: perspectives and opportunities
Grain quality improvement is a key target for rice breeders, along with yield. It is a multigenic
trait that is simultaneously influenced by many factors. Over the past few decades, breeding …
trait that is simultaneously influenced by many factors. Over the past few decades, breeding …
Self-tunable engineered yeast probiotics for the treatment of inflammatory bowel disease
Inflammatory bowel disease (IBD) is a complex chronic inflammatory disorder of the
gastrointestinal tract. Extracellular adenosine triphosphate (eATP) produced by the …
gastrointestinal tract. Extracellular adenosine triphosphate (eATP) produced by the …
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
M Haeussler, K Schönig, H Eckert, A Eschstruth… - Genome biology, 2016 - Springer
Background The success of the CRISPR/Cas9 genome editing technique depends on the
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
Dual-AAV delivering split prime editor system for in vivo genome editing
S Zhi, Y Chen, G Wu, J Wen, J Wu, Q Liu, Y Li, R Kang… - Molecular Therapy, 2022 - cell.com
Prime editor (PE), a new genome editing tool, can generate all 12 possible base-to-base
conversions, insertion, and deletion of short fragment DNA. PE has the potential to correct …
conversions, insertion, and deletion of short fragment DNA. PE has the potential to correct …
Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation
Amyotrophic lateral sclerosis (ALS) is the most frequent motor neuron disease. Cytoplasmic
fused in sarcoma (FUS) aggregates are pathological hallmarks of FUS-ALS. Proper shuttling …
fused in sarcoma (FUS) aggregates are pathological hallmarks of FUS-ALS. Proper shuttling …
Human sensorimotor organoids derived from healthy and amyotrophic lateral sclerosis stem cells form neuromuscular junctions
JD Pereira, DM DuBreuil, AC Devlin, A Held… - Nature …, 2021 - nature.com
Human induced pluripotent stem cells (iPSC) hold promise for modeling diseases in
individual human genetic backgrounds and thus for developing precision medicine. Here …
individual human genetic backgrounds and thus for developing precision medicine. Here …
GuideScan software for improved single and paired CRISPR guide RNA design
We present GuideScan software for the design of CRISPR guide RNA libraries that can be
used to edit coding and noncoding genomic regions. GuideScan produces high-density sets …
used to edit coding and noncoding genomic regions. GuideScan produces high-density sets …
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
DM Sapozhnikov, M Szyf - Nature Communications, 2021 - nature.com
Despite four decades of research to support the association between DNA methylation and
gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that …
gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that …
[HTML][HTML] Prime editing: current advances and therapeutic opportunities in human diseases
Gene editing ushers in a new era of disease treatment since many genetic diseases are
caused by base-pair mutations in genomic DNA. With the rapid development of genome …
caused by base-pair mutations in genomic DNA. With the rapid development of genome …