Nuclear compartmentalization as a mechanism of quantitative control of gene expression

P Bhat, D Honson, M Guttman - Nature Reviews Molecular Cell Biology, 2021 - nature.com
Gene regulation requires the dynamic coordination of hundreds of regulatory factors at
precise genomic and RNA targets. Although many regulatory factors have specific affinity for …

Plants use molecular mechanisms mediated by biomolecular condensates to integrate environmental cues with development

S Field, GJ Jang, C Dean, LC Strader, SY Rhee - The Plant Cell, 2023 - academic.oup.com
This review highlights recent literature on biomolecular condensates in plant development
and discusses challenges for fully dissecting their functional roles. Plant developmental …

Fixation can change the appearance of phase separation in living cells

S Irgen-Gioro, S Yoshida, V Walling, S Chong - elife, 2022 - elifesciences.org
Fixing cells with paraformaldehyde (PFA) is an essential step in numerous biological
techniques as it is thought to preserve a snapshot of biomolecular transactions in living cells …

Direct Observation of “Elongated” Conformational States in α‐Synuclein upon Liquid‐Liquid Phase Separation

D Ubbiali, M Fratini, L Piersimoni, CH Ihling… - Angewandte …, 2022 - Wiley Online Library
Abstract α‐Synuclein (α‐syn) is an intrinsically disordered protein (IDP) that undergoes
liquid‐liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy …

Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation

A Avni, A Joshi, A Walimbe, SG Pattanashetty… - Nature …, 2022 - nature.com
Biomolecular condensates formed via liquid-liquid phase separation (LLPS) are involved in
a myriad of critical cellular functions and debilitating neurodegenerative diseases …

RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding

E Zacco, L Broglia, M Kurihara, M Monti… - Chemical …, 2024 - ACS Publications
This comprehensive Review delves into the chemical principles governing RNA-mediated
crowding events, commonly referred to as granules or biological condensates. We explore …

[HTML][HTML] It's just a phase: exploring the relationship between mRNA, biomolecular condensates, and translational control

DM Parker, LP Winkenbach… - Frontiers in …, 2022 - frontiersin.org
Cells spatially organize their molecular components to carry out fundamental biological
processes and guide proper development. The spatial organization of RNA within the cell …

Protein phase separation in plant membrane biology: more than just a compartmentalization strategy

JM Dragwidge, D Van Damme - The Plant Cell, 2023 - academic.oup.com
The formation of biomolecular condensates through phase separation is an important
strategy to compartmentalize cellular functions. While it is now well established that …

Advanced surface passivation for high-sensitivity studies of biomolecular condensates

RW Yao, MK Rosen - … of the National Academy of Sciences, 2024 - National Acad Sciences
Biomolecular condensates are cellular compartments that concentrate biomolecules without
an encapsulating membrane. In recent years, significant advances have been made in the …

Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping

G Foran, RD Hallam, M Megaly, A Turgambayeva… - Scientific Reports, 2024 - nature.com
The Notch receptor is a pleiotropic signaling protein that translates intercellular ligand
interactions into changes in gene expression via the nuclear localization of the Notch …