Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthesis of dopamine
(DA) and other catecholamines, and its dysfunction leads to DA deficiency and …

Xmipp is a specialized software package for image processing in electron microscopy, and
that is mainly focused on 3D reconstruction of macromolecules through single-particles …

Polypeptides and polynucleotides are natural programmable biopolymers that can self-
assemble into complex tertiary structures. We describe a system analogous to designed …

J-domain proteins tune the specificity of Hsp70s, engaging them in precise functions.
Despite their essential role, the structure and function of many J-domain proteins remain …

Recently, single-particle cryo-electron microscopy (cryo-EM) has become an indispensable
method for determining macromolecular structures at high resolution to deeply explore the …

Faithful chromosome segregation depends on the ability of sister kinetochores to attach to
spindle microtubules. The outer layer of kinetochores transiently expands in early mitosis to …

Mitochondria are responsible for energy production through aerobic respiration, and
represent the powerhouse of eukaryotic cells. Their metabolism and gene expression …

Complex biological processes are often performed by self-organizing nanostructures
comprising multiple classes of macromolecules, such as ribosomes (proteins and RNA) or …

Aerobic methane oxidation is catalyzed by particulate methane monooxygenase (pMMO), a
copper-dependent, membrane metalloenzyme composed of subunits PmoA, PmoB, and …