Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

This critical review provides an overview of the continually expanding family of fluorescent
proteins (FPs) that have become essential tools for studies of cell biology and physiology …

Genetically encoded calcium indicators (GECIs) are powerful tools for systems
neuroscience. Here we describe red, single-wavelength GECIs,“RCaMPs,” engineered from …

Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We
developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and …

Dynamic protein interactions play a significant part in many cellular processes. A technique
that shows considerable promise in elucidating such interactions is Förster resonance …

Current fluorescent protein (FP) development strategies are focused on fine-tuning the
photophysical properties of blue to yellow variants derived from the Aequorea victoria …

Fluorescence has long been recognized as a powerful tool for probing biological structure
and function. Nearly sixty-five years ago, Coons and colleagues introduced the first …

Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores
in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely …

Ryo Iizuka, Mai Yamagishi-Shirasaki, Takashi Funatsu. Green fluorescent protein (GFP) has
a chromophore that forms autocatalytically within the folded protein. Although many studies …

The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on
brightness and photostability, which are dependent on the characteristics of the fluorescent …