Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

Filament formation by non-cytoskeletal enzymes has been known for decades, yet only
relatively recently has its wide-spread role in enzyme regulation and biology come to be …

The Drosophila genome contains> 13000 protein-coding genes, the majority of which
remain poorly investigated. Important reasons include the lack of antibodies or reporter …

Proper differentiation of sperm from germline stem cells, essential for production of the next
generation, requires dramatic changes in gene expression that drive remodeling of almost …

During animal development, the spatiotemporal properties of molecular events largely
determine the biological outcomes. Conventional gene analysis methods lack the …

Noncentrosomal microtubules play an important role in polarizing differentiated cells, but
little is known about how these microtubules are organized. Here we identify the …

Previously, we described a large collection of Minos-Mediated Integration Cassettes
(MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a …

Apical–basal polarity is essential for the formation and function of epithelial tissues, whereas
loss of polarity is a hallmark of tumours. Studies in Drosophila have identified conserved …

α-Catenin couples the cadherin–catenin complex to the actin cytoskeleton. The
mechanosensitive α-Catenin M region undergoes conformational changes upon application …

The role of protein localization along the apical-basal axis of polarized cells is difficult to
investigate in vivo, partially due to lack of suitable tools. Here, we present the GrabFP …