Recent advances in isobaric labeling and applications in quantitative proteomics

MK Sivanich, TJ Gu, DN Tabang, L Li - Proteomics, 2022 - Wiley Online Library
Mass spectrometry (MS) has emerged at the forefront of quantitative proteomic techniques.
Liquid chromatography‐mass spectrometry (LC‐MS) can be used to determine abundances …

6-Plex mdSUGAR Isobaric-Labeling Guide Fingerprint Embedding for Glycomics Analysis

M Ma, M Li, Y Zhu, Y Zhao, F Wu, Z Wang… - Analytical …, 2023 - ACS Publications
Glycans are vital biomolecules with diverse functions in biological processes. Mass
spectrometry (MS) has become the most widely employed technology for glycomics studies …

High-throughput quantitative glycomics enabled by 12-plex isobaric multiplex labeling reagents for carbonyl-containing compound (SUGAR) tags

M Li, Y Feng, M Ma, A Kapur… - Journal of proteome …, 2023 - ACS Publications
Glycans, which are widely distributed on most proteins and cell surfaces, are a class of
important biomolecules playing crucial roles in various biological processes such as …

Recent Advances in Labeling‐Based Quantitative Glycomics: From High‐Throughput Quantification to Structural Elucidation

Z Wang, J Zhang, L Li - Proteomics, 2025 - Wiley Online Library
Glycosylation, a crucial posttranslational modification (PTM), plays important roles in
numerous biological processes and is linked to various diseases. Despite its significance …

Development of novel isobaric tags enables accurate and sensitive multiplexed proteomics using complementary ions

M Li, M Ma, L Li - Analytical and bioanalytical chemistry, 2023 - Springer
High-throughput quantitative analysis of the cells' proteomes across multiple conditions such
as various perturbations and different time points is essential for gaining insights into …

Carboxyl-based CPMP tag for ultrasensitive analysis of disaccharides by negative tandem mass spectrometry

S Ma, F Chen, M Zhang, H Yuan, G Ouyang… - Analytical …, 2022 - ACS Publications
Here, we develop a sensitive method for glucose-containing disaccharide analysis by 1-(4-
carboxyphenyl)-3-methyl-5-pyrazolone (CPMP) derivatization using mass spectrometry. The …

Novel isobaric tagging reagent enabled multiplex quantitative glycoproteomics via electron-transfer/higher-energy collisional dissociation (EThcD) mass spectrometry

M Li, X Zhong, Y Feng, L Li - Journal of the American Society for …, 2022 - ACS Publications
Protein glycosylation, covalent attachment of carbohydrates to polypeptide chains, is a
highly important post-translational modification involved in many essential physiological …

GlycoTCFM: Glycoproteomics Based on Two Complementary Fragmentation Methods Reveals Distinctive O-Glycosylation in Human Sperm and Seminal Plasma

M Luo, T Su, Q Cheng, X Zhang, F Cai… - Journal of Proteome …, 2023 - ACS Publications
Human semen, consisting of spermatozoa (sperm) and seminal plasma, represents a
special clinical sample type in human body fluid. Protein glycosylation in sperm and seminal …