Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

For quantification of gene-specific mRNA, quantitative real-time RT-PCR has become one of
the most frequently used methods over the last few years. This article focuses on the issue of …

Background The 2-ΔΔ CT method has been extensively used as a relative quantification
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …

Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA
transcripts, most analyses of qPCR data are still delegated to the software that comes with …

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the
evident requirement for quantitative data analysis in molecular medicine, biotechnology …

This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …

Liver steatosis is one of the main drivers for the development of whole-body insulin
resistance. Conversely, aerobic training (AT) has been suggested as non-pharmacological …

Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of
choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful …

qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection
and quantification of RNA targets and is being utilized increasingly in novel clinical …

Real‐time PCR is being used increasingly as the method of choice for mRNA quantification,
allowing rapid analysis of gene expression from low quantities of starting template. Despite …