In situ cryo-electron tomography: a post-reductionist approach to structural biology
Cryo-electron tomography is a powerful technique that can faithfully image the native
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo …
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo …
[HTML][HTML] Visualization of translation and protein biogenesis at the ER membrane
M Gemmer, ML Chaillet, J van Loenhout… - Nature, 2023 - nature.com
The dynamic ribosome–translocon complex, which resides at the endoplasmic reticulum
(ER) membrane, produces a major fraction of the human proteome,. It governs the synthesis …
(ER) membrane, produces a major fraction of the human proteome,. It governs the synthesis …
Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging
FKM Schur - Current opinion in structural biology, 2019 - Elsevier
Highlights•Cryo-ET and subtomogram averaging allow visualizing macromolecular
complexes in close to physiological conditions.•This offers a significant potential for in situ …
complexes in close to physiological conditions.•This offers a significant potential for in situ …
Extensive angular sampling enables the sensitive localization of macromolecules in electron tomograms
Cryo-electron tomography provides 3D images of macromolecules in their cellular context.
To detect macromolecules in tomograms, template matching (TM) is often used, which uses …
To detect macromolecules in tomograms, template matching (TM) is often used, which uses …
Fast and accurate reference-free alignment of subtomograms
In cryoelectron tomography alignment and averaging of subtomograms, each dnepicting the
same macromolecule, improves the resolution compared to the individual subtomogram …
same macromolecule, improves the resolution compared to the individual subtomogram …
A convolutional autoencoder approach for mining features in cellular electron cryo-tomograms and weakly supervised coarse segmentation
X Zeng, MR Leung, T Zeev-Ben-Mordehai… - Journal of structural …, 2018 - Elsevier
Cellular electron cryo-tomography enables the 3D visualization of cellular organization in
the near-native state and at submolecular resolution. However, the contents of cellular …
the near-native state and at submolecular resolution. However, the contents of cellular …
Deep learning-based subdivision approach for large scale macromolecules structure recovery from electron cryo tomograms
Abstract Motivation Cellular Electron CryoTomography (CECT) enables 3D visualization of
cellular organization at near-native state and in sub-molecular resolution, making it a …
cellular organization at near-native state and in sub-molecular resolution, making it a …
SHREC 2020: Classification in cryo-electron tomograms
I Gubins, ML Chaillet, G van Der Schot… - Computers & …, 2020 - Elsevier
Cryo-electron tomography (cryo-ET) is an imaging technique that allows us to three-
dimensionally visualize both the structural details of macro-molecular assemblies under …
dimensionally visualize both the structural details of macro-molecular assemblies under …
In situ structural studies of tripeptidyl peptidase II (TPPII) reveal spatial association with proteasomes
Y Fukuda, F Beck, JM Plitzko… - Proceedings of the …, 2017 - National Acad Sciences
Tripeptidyl peptidase II (TPPII) is a eukaryotic protease acting downstream of the 26S
proteasome; it removes tripeptides from the degradation products released by the …
proteasome; it removes tripeptides from the degradation products released by the …
Cryo-electron tomography
J Plitzko, WP Baumeister - Springer handbook of microscopy, 2019 - Springer
Classical structural biology approaches rely on highly purified molecules which are isolated
from their neighbors far from the complex macromolecular interaction network of the cell (ex …
from their neighbors far from the complex macromolecular interaction network of the cell (ex …