Autofocusing technologies for whole slide imaging and automated microscopy

Z Bian, C Guo, S Jiang, J Zhu, R Wang… - Journal of …, 2020 - Wiley Online Library
Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in
recent years. Due to the inherent tissue topography variability, accurate autofocusing …

Fluorescence Microscopy: a statistics-optics perspective

M Fazel, KS Grussmayer, B Ferdman, A Radenovic… - Reviews of Modern …, 2024 - APS
Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz

T Nöbauer, Y Zhang, H Kim, A Vaziri - Nature methods, 2023 - nature.com
Various implementations of mesoscopes provide optical access for calcium imaging across
multi-millimeter fields of view in the mammalian brain; however, capturing the activity of the …

Multifocus microscopy with optical sectioning and high axial resolution

F Ströhl, DH Hansen, M Nager Grifo, ÅB Birgisdottir - Optica, 2022 - opg.optica.org
Multifocus microscopy enables recording of entire volumes in a single camera exposure. In
dense samples, multifocus microscopy is severely hampered by background haze. Here, we …

[HTML][HTML] KHz-rate volumetric voltage imaging of the whole Zebrafish heart

L Sacconi, L Silvestri, EC Rodríguez, GAB Armstrong… - Biophysical Reports, 2022 - cell.com
Fast volumetric imaging is essential for understanding the function of excitable tissues such
as those found in the brain and heart. Measuring cardiac voltage transients in tissue …

Tandem aberration correction optics (TACO) in wide-field structured illumination microscopy

D Gong, NF Scherer - Biomedical Optics Express, 2023 - opg.optica.org
Structured illumination microscopy (SIM) is a powerful super-resolution imaging technique
that uses patterned illumination to down-modulate high spatial-frequency information of …

Fast, multiplane line-scan confocal microscopy using axially distributed slits

JM Tsang, HJ Gritton, SL Das, TD Weber… - Biomedical Optics …, 2021 - opg.optica.org
The inherent constraints on resolution, speed and field of view have hindered the
development of high-speed, three-dimensional microscopy techniques over large scales …

Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency

KD Mac, MM Qureshi, M Na, S Chang, TJ Eom… - Optics …, 2022 - opg.optica.org
In microscopic imaging of biological tissues, particularly real-time visualization of neuronal
activities, rapid acquisition of volumetric images poses a prominent challenge. Typically, two …

The autism spectrum disorder risk gene NEXMIF over-synchronizes hippocampal CA1 network and alters neuronal coding

RA Mount, M Athif, M O'Connor, A Saligrama… - Frontiers in …, 2023 - frontiersin.org
Mutations in autism spectrum disorder (ASD) risk genes disrupt neural network dynamics
that ultimately lead to abnormal behavior. To understand how ASD-risk genes influence …

Squid: simplifying quantitative imaging platform development and deployment

H Li, D Krishnamurthy, E Li, P Vyas, N Akireddy, C Chai… - bioRxiv, 2020 - biorxiv.org
With rapid developments in microscopy methods, highly versatile, robust and affordable
implementations are needed to enable rapid and wide adoption by the biological sciences …