The removal of introns from mRNA precursors and its regulation by alternative splicing are
key for eukaryotic gene expression and cellular function, as evidenced by the numerous …

The spliceosome removes introns from messenger RNA precursors (pre-mRNA). Decades
of biochemistry and genetics combined with recent structural studies of the spliceosome …

Sequence logos are visually compelling ways of illustrating the biological properties of DNA,
RNA and protein sequences, yet it is currently difficult to generate and customize such logos …

Multiplex assays of variant effect (MAVEs), such as deep mutational scans and massively
parallel reporter assays, test thousands of sequence variants in a single experiment. Despite …

Scalable sequence–function studies have enabled the systematic analysis and cataloging of
hundreds of thousands of coding and noncoding genetic variants in the human genome …

Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.
Here, we describe a method to harness this abnormal splicing activity to drive splicing factor …

Over the last decade, a rich variety of massively parallel assays have revolutionized our
understanding of how biological sequences encode quantitative molecular phenotypes …

Alternative splicing of messenger RNAs is associated with the evolution of developmentally
complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA …

Multiplex assays of variant effect (MAVEs) are a family of methods that includes deep
mutational scanning experiments on proteins and massively parallel reporter assays on …

Many nascent long non-coding RNAs (lncRNAs) undergo the same maturation steps as pre-
mRNAs of protein-coding genes (PCGs), but they are often poorly spliced. To identify the …