Recombinant biotherapeutic proteins such as monoclonal antibodies are mostly produced in
Chinese hamster ovary (CHO) cells and pharmaceutical companies are interested in an …

334 SAIER ET AL. ally similar (see sections III and V). Cleavage of amino-terminal signal
sequences by signal peptidases occurs with similar specificities (89, 184, 220, 322); the …

Much progress has been made in recent years regarding the mechanisms of targeting of
secretory proteins to, and across, the endoplasmic reticulum (ER) membrane. Many of the …

The environment of secretory proteins undergoing translocation across the ER membrane
was determlned by Incorporating fluorescent probes Into nascent chains during translatlon …

The signal sequence is in an aqueous milieu at an early stage in the translocation of a
nascent secretory protein across the endoplasmic reticulum membrane. This was …

Numerous proteins targeted for the secretory pathway are increasingly implicated in
functional or pathological roles at alternative cellular destinations. The parameters that allow …

Abstract Background Signal peptides (SPs) mediate the targeting of secretory precursor
proteins to the correct subcellular compartments in prokaryotes and eukaryotes. Identifying …

Like all other secretory proteins, the HIV-1 envelope glycoprotein gp160 is targeted to the
endoplasmic reticulum (ER) by its signal peptide during synthesis. Proper gp160 folding in …

Many eukaryotic proteins are modified by N-linked glycosylation, a process in which
oligosaccharides are added to asparagine residues in the sequon Asn-X-Ser/Thr. However …

Signal peptides (SPs) direct nascent secretory and membrane proteins to the membrane of
the endoplasmic reticulum. They are usually cleaved from the nascent polypeptide by signal …