Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival
Recent physiological and ecological studies have challenged the long-held belief that
microbial metabolism of molecular hydrogen (H2) is a niche process. To gain a broader …
microbial metabolism of molecular hydrogen (H2) is a niche process. To gain a broader …
Proton transfer in the catalytic cycle of [NiFe] hydrogenases: insight from vibrational spectroscopy
PA Ash, R Hidalgo, KA Vincent - ACS catalysis, 2017 - ACS Publications
Catalysis of H2 production and oxidation reactions is critical in renewable energy systems
based around H2 as a clean fuel, but the present reliance on platinum-based catalysts is not …
based around H2 as a clean fuel, but the present reliance on platinum-based catalysts is not …
Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases
The comparison of homologous metalloenzymes, in which the same inorganic active site is
surrounded by a variable protein matrix, has demonstrated that residues that are remote …
surrounded by a variable protein matrix, has demonstrated that residues that are remote …
Reversible glutamate coordination to high-valent nickel protects the active site of a [NiFe] hydrogenase from oxygen
NAD+-reducing [NiFe] hydrogenases are valuable biocatalysts for H2-based energy
conversion and the regeneration of nucleotide cofactors. While most hydrogenases are …
conversion and the regeneration of nucleotide cofactors. While most hydrogenases are …
Oxygen-Tolerant H2 Production by [FeFe]-H2ase Active Site Mimics Aided by Second Sphere Proton Shuttle
The instability of [FeFe]-H2ases and their biomimetics toward O2 renders them inefficient to
implement in practical H2 generation (HER). Previous investigations on synthetic models as …
implement in practical H2 generation (HER). Previous investigations on synthetic models as …
Oxygen-tolerant proton reduction catalysis: much O 2 about nothing?
DW Wakerley, E Reisner - Energy & Environmental Science, 2015 - pubs.rsc.org
Proton reduction catalysts are an integral component of artificial photosynthetic systems for
the production of H2. This perspective covers such catalysts with respect to their tolerance …
the production of H2. This perspective covers such catalysts with respect to their tolerance …
Ultrafast 2D-IR spectroscopy of [NiFe] hydrogenase from E. coli reveals the role of the protein scaffold in controlling the active site environment
SLD Wrathall, B Procacci, M Horch, E Saxton… - Physical Chemistry …, 2022 - pubs.rsc.org
Ultrafast two-dimensional infrared (2D-IR) spectroscopy of Escherichia coli Hyd-1 (EcHyd-1)
reveals the structural and dynamic influence of the protein scaffold on the Fe (CO)(CN) 2 unit …
reveals the structural and dynamic influence of the protein scaffold on the Fe (CO)(CN) 2 unit …
Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy
M Horch, J Schoknecht, SLD Wrathall… - Chemical …, 2019 - pubs.rsc.org
Hydrogenases are valuable model enzymes for sustainable energy conversion approaches
using H2, but rational utilization of these base-metal biocatalysts requires a detailed …
using H2, but rational utilization of these base-metal biocatalysts requires a detailed …
O2-tolerant [NiFe]-hydrogenases of Ralstonia eutropha H16: Physiology, molecular biology, purification, and biochemical analysis
Abstract Dioxygen-tolerant [NiFe]-hydrogenases are defined by their ability to catalyze the
reaction, H 2⇌ 2H++ 2e− even in the presence of O 2. Catalytic and probably also …
reaction, H 2⇌ 2H++ 2e− even in the presence of O 2. Catalytic and probably also …
Comprehensive reaction mechanisms at and near the Ni–Fe active sites of [NiFe] hydrogenases
[NiFe] hydrogenase (H2ase) catalyzes the oxidation of dihydrogen to two protons and two
electrons and/or its reverse reaction. For this simple reaction, the enzyme has developed a …
electrons and/or its reverse reaction. For this simple reaction, the enzyme has developed a …