[HTML][HTML] New synthetic biology tools for metabolic control

X Lv, A Hueso-Gil, X Bi, Y Wu, Y Liu, L Liu… - Current Opinion in …, 2022 - Elsevier
In industrial bioprocesses, microbial metabolism dictates the product yields, and therefore,
our capacity to control it has an enormous potential to help us move towards a bio-based …

Direct cell reprogramming for tissue engineering and regenerative medicine

A Grath, G Dai - Journal of biological engineering, 2019 - Springer
Direct cell reprogramming, also called transdifferentiation, allows for the reprogramming of
one somatic cell type directly into another, without the need to transition through an induced …

[HTML][HTML] Context-aware synthetic biology by controller design: Engineering the mammalian cell

N Shakiba, RD Jones, R Weiss, D Del Vecchio - Cell systems, 2021 - cell.com
The rise of systems biology has ushered a new paradigm: the view of the cell as a system
that processes environmental inputs to drive phenotypic outputs. Synthetic biology provides …

A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivo

G Zhong, H Wang, W He, Y Li, H Mou, ZJ Tickner… - Nature …, 2020 - nature.com
Widespread use of gene therapy technologies is limited in part by the lack of small genetic
switches with wide dynamic ranges that control transgene expression without the …

Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production

KJ Lauersen - Planta, 2019 - Springer
Main conclusions Eukaryotic microalgae hold incredible metabolic potential for the
sustainable production of heterologous isoprenoid products. Recent advances in algal …

Rational engineering of synthetic microbial systems: from single cells to consortia

P Bittihn, MO Din, LS Tsimring, J Hasty - Current opinion in microbiology, 2018 - Elsevier
Highlights•Synthetic biology approaches enable microbial systems engineering.•Versatile
tools rapidly expand regulatory engineering capabilities.•Modular components and …

Combinatorial metabolic pathway assembly approaches and toolkits for modular assembly

R Young, M Haines, M Storch, PS Freemont - Metabolic Engineering, 2021 - Elsevier
Synthetic Biology is a rapidly growing interdisciplinary field that is primarily built upon
foundational advances in molecular biology combined with engineering design principles …

High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis

DM Mason, CR Weber, C Parola, SM Meng… - Nucleic acids …, 2018 - academic.oup.com
Antibody engineering is often performed to improve therapeutic properties by directed
evolution, usually by high-throughput screening of phage or yeast display libraries …

Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells

AM Makri Pistikou, GAO Cremers, BL Nathalia… - Nature …, 2023 - nature.com
The rational design and implementation of synthetic mammalian communication systems
can unravel fundamental design principles of cell communication circuits and offer a …

Cell line techniques and gene editing tools for antibody production: a review

AK Dangi, R Sinha, S Dwivedi, SK Gupta… - Frontiers in …, 2018 - frontiersin.org
The present day modern formulation practices for drugs are based on newer tools and
techniques toward effective utilization. The methods of antibody formulations are to be …