Best practices for single-cell analysis across modalities

L Heumos, AC Schaar, C Lance, A Litinetskaya… - Nature Reviews …, 2023 - nature.com
Recent advances in single-cell technologies have enabled high-throughput molecular
profiling of cells across modalities and locations. Single-cell transcriptomics data can now …

Characterizing cis-regulatory elements using single-cell epigenomics

S Preissl, KJ Gaulton, B Ren - Nature Reviews Genetics, 2023 - nature.com
Cell type-specific gene expression patterns and dynamics during development or in disease
are controlled by cis-regulatory elements (CREs), such as promoters and enhancers …

SCENIC+: single-cell multiomic inference of enhancers and gene regulatory networks

C Bravo González-Blas, S De Winter, G Hulselmans… - Nature …, 2023 - nature.com
Joint profiling of chromatin accessibility and gene expression in individual cells provides an
opportunity to decipher enhancer-driven gene regulatory networks (GRNs). Here we present …

Multi-omics single-cell data integration and regulatory inference with graph-linked embedding

ZJ Cao, G Gao - Nature Biotechnology, 2022 - nature.com
Despite the emergence of experimental methods for simultaneous measurement of multiple
omics modalities in single cells, most single-cell datasets include only one modality. A major …

Single-cell chromatin state analysis with Signac

T Stuart, A Srivastava, S Madad, CA Lareau, R Satija - Nature methods, 2021 - nature.com
The recent development of experimental methods for measuring chromatin state at single-
cell resolution has created a need for computational tools capable of analyzing these …

Comparative cellular analysis of motor cortex in human, marmoset and mouse

TE Bakken, NL Jorstad, Q Hu, BB Lake, W Tian… - Nature, 2021 - nature.com
The primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally
conserved across mammals. Here, using high-throughput transcriptomic and epigenomic …

A multimodal cell census and atlas of the mammalian primary motor cortex

Principal manuscript editors, Analysis coordination… - Nature, 2021 - nature.com
Here we report the generation of a multimodal cell census and atlas of the mammalian
primary motor cortex as the initial product of the BRAIN Initiative Cell Census Network …

Neutrophils and emergency granulopoiesis drive immune suppression and an extreme response endotype during sepsis

AJ Kwok, A Allcock, RC Ferreira, E Cano-Gamez… - Nature …, 2023 - nature.com
Sepsis arises from diverse and incompletely understood dysregulated host response
processes following infection that leads to life-threatening organ dysfunction. Here we …

Single-nucleus chromatin accessibility and transcriptomic characterization of Alzheimer's disease

S Morabito, E Miyoshi, N Michael, S Shahin… - Nature …, 2021 - nature.com
The gene-regulatory landscape of the brain is highly dynamic in health and disease,
coordinating a menagerie of biological processes across distinct cell types. Here, we …

[HTML][HTML] A single-cell atlas of chromatin accessibility in the human genome

K Zhang, JD Hocker, M Miller, X Hou, J Chiou… - Cell, 2021 - cell.com
Current catalogs of regulatory sequences in the human genome are still incomplete and lack
cell type resolution. To profile the activity of gene regulatory elements in diverse cell types …