A review on macroscale and microscale cell lysis methods

M Shehadul Islam, A Aryasomayajula… - Micromachines, 2017 - mdpi.com
The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit
operation in biomolecular analysis. This paper presents a critical evaluation of the various …

[PDF][PDF] A threshold selection method from gray-level histograms

N Otsu - Automatica, 1975 - dspace.tul.cz
Summary 16S rRNA-targeted oligonucleotide probes for eubacteria (EUB338), ammonium-
oxidizing bacteria (Nsm156) and nitrite-oxidizing bacteria (Nb1000) were used for the rapid …

Complete nitrification by a single microorganism

MAHJ Van Kessel, DR Speth, M Albertsen, PH Nielsen… - Nature, 2015 - nature.com
Nitrification is a two-step process where ammonia is first oxidized to nitrite by ammonia-
oxidizing bacteria and/or archaea, and subsequently to nitrate by nitrite-oxidizing bacteria …

Full scale performance of the aerobic granular sludge process for sewage treatment

M Pronk, MK De Kreuk, B De Bruin, P Kamminga… - Water research, 2015 - Elsevier
Recently, aerobic granular sludge technology has been scaled-up and implemented for
industrial and municipal wastewater treatment under the trade name Nereda®. With full …

Phylogenetic identification and in situ detection of individual microbial cells without cultivation

RI Amann, W Ludwig, KH Schleifer - Microbiological reviews, 1995 - Am Soc Microbiol
The frequent discrepancy between direct microscopic counts and numbers of culturable
bacteria from environmental samples is just one of several indications that we currently …

Predominant archaea in marine sediments degrade detrital proteins

KG Lloyd, L Schreiber, DG Petersen, KU Kjeldsen… - Nature, 2013 - nature.com
Half of the microbial cells in the Earth's oceans are found in sediments. Many of these cells
are members of the Archaea, single-celled prokaryotes in a domain of life separate from …

Zero-valent sulphur is a key intermediate in marine methane oxidation

J Milucka, TG Ferdelman, L Polerecky, D Franzke… - Nature, 2012 - nature.com
Emissions of methane, a potent greenhouse gas, from marine sediments are controlled by
anaerobic oxidation of methane coupled primarily to sulphate reduction (AOM). Sulphate …

[HTML][HTML] Developmental microbial ecology of the neonatal gastrointestinal tract

RI Mackie, A Sghir, HR Gaskins - The American journal of clinical nutrition, 1999 - Elsevier
The gastrointestinal tract of a normal fetus is sterile. During the birth process and rapidly
thereafter, microbes from the mother and surrounding environment colonize the …

Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions

W Manz, R Amann, W Ludwig, M Wagner… - Systematic and applied …, 1992 - Elsevier
Based on comparative analyses of 16S and 23S ribosomal RNA sequences we have
located sites specific for the alpha-, beta-, and gamma-subclasses of Proteobacteria. Short …

Optimizing fluorescent in situ hybridization with rRNA‐targeted oligonucleotide probes for flow cytometric identification of microorganisms

G Wallner, R Amann, W Beisker - Cytometry: The Journal of the …, 1993 - Wiley Online Library
A combination of fluorescent rRNA‐targeted oligonucleotide probes (“phylogenetic stains”)
and flow cytometry was used for a high resolution automated analysis of mixed microbial …