Genetically encodable fluorescent and bioluminescent biosensors light up signaling networks

X Zhou, S Mehta, J Zhang - Trends in biochemical sciences, 2020 - cell.com
Cell signaling networks are intricately regulated in time and space to determine the
responses and fates of cells to different cues. Genetically encodable fluorescent and …

Fluorescent biosensors illuminate the spatial regulation of cell signaling across scales

AC Lyons, S Mehta, J Zhang - Biochemical Journal, 2023 - portlandpress.com
As cell signaling research has advanced, it has become clearer that signal transduction has
complex spatiotemporal regulation that goes beyond foundational linear transduction …

Orthogonal fluorescent chemogenetic reporters for multicolor imaging

AG Tebo, B Moeyaert, M Thauvin… - Nature chemical …, 2021 - nature.com
Spectrally separated fluorophores allow the observation of multiple targets simultaneously
inside living cells, leading to a deeper understanding of the molecular interplay that …

3D super-resolution optical fluctuation imaging with temporal focusing two-photon excitation

P Szczypkowski, M Pawlowska… - Biomedical Optics …, 2024 - opg.optica.org
3D super-resolution fluorescence microscopy typically requires sophisticated setups,
sample preparation, or long measurements. A notable exception, SOFI, only requires …

Correcting for photodestruction in super-resolution optical fluctuation imaging

Y Peeters, W Vandenberg, S Duwé, A Bouwens… - Scientific reports, 2017 - nature.com
Super-resolution optical fluctuation imaging overcomes the diffraction limit by analyzing
fluctuations in the fluorophore emission. A key assumption of the imaging is that the …

Super-resolution imaging of plasmonic near-fields: overcoming emitter mislocalizations

Y Miao, RC Boutelle, A Blake… - The Journal of …, 2022 - ACS Publications
Plasmonic nano-objects have shown great potential in enhancing applications like
biological/chemical sensing, light harvesting and energy transfer, and optical/quantum …

GMars-T enabling multimodal subdiffraction structural and functional fluorescence imaging in live cells

S Wang, X Chen, L Chang, M Ding, R Xue… - Analytical …, 2018 - ACS Publications
Fluorescent probes with multimodal and multilevel imaging capabilities are highly valuable
as imaging with such probes not only can obtain new layers of information but also enable …

Molecular dynamic indicators of the photoswitching properties of green fluorescent proteins

D Smyrnova, B Moeyaert, S Michielssens… - The Journal of …, 2015 - ACS Publications
Reversibly photoswitchable fluorescent proteins (RSFPs) are highly useful probes for a
range of applications including diffraction-unlimited fluorescence microscopy. It was …

Thermal isomerization mechanism in Dronpa and its mutants

D Smyrnova, K Zinovjev, I Tunon… - The Journal of Physical …, 2016 - ACS Publications
The photoswitching speed of the reversibly switchable fluorescent proteins (RSFPs) from the
family of green fluorescent proteins (GFPs) changes upon mutation which is of direct …

Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry

TYH Bui, E De Zitter, B Moeyaert, L Pecqueur… - International Journal of …, 2023 - Elsevier
Reversibly switchable monomeric Cherry (rsCherry) is a photoswitchable variant of the red
fluorescent protein mCherry. We report that this protein gradually and irreversibly loses its …