Metabolic engineering for the synthesis of polyesters: A 100-year journey from polyhydroxyalkanoates to non-natural microbial polyesters

SY Choi, MN Rhie, HT Kim, JC Joo, IJ Cho, J Son… - Metabolic …, 2020 - Elsevier
As concerns increase regarding sustainable industries and environmental pollutions caused
by the accumulation of non-degradable plastic wastes, bio-based polymers, particularly …

New insights in the formation of polyhydroxyalkanoate granules (carbonosomes) and novel functions of poly (3‐hydroxybutyrate)

D Jendrossek, D Pfeiffer - Environmental microbiology, 2014 - Wiley Online Library
The metabolism of polyhydroxybutyrate (PHB) and related polyhydroxyalkanoates (PHAs)
has been investigated by many groups for about three decades, and good progress was …

Structural insights into polyhydroxyalkanoates biosynthesis

HY Sagong, HF Son, SY Choi, SY Lee… - Trends in biochemical …, 2018 - cell.com
Polyhydroxyalkanoates (PHAs) are diverse biopolyesters produced by numerous
microorganisms and have attracted much attention as a substitute for petroleum-based …

Polyhydroxyalkanoate (PHA) granules have no phospholipids

S Bresan, A Sznajder, W Hauf, K Forchhammer… - Scientific reports, 2016 - nature.com
Polyhydroxybutyrate (PHB) granules, also designated as carbonosomes, are supra-
molecular complexes in prokaryotes consisting of a PHB polymer core and a surface layer of …

Phasins, multifaceted polyhydroxyalkanoate granule-associated proteins

MP Mezzina, MJ Pettinari - Applied and environmental …, 2016 - Am Soc Microbiol
Phasins are the major polyhydroxyalkanoate (PHA) granule-associated proteins. They
promote bacterial growth and PHA synthesis and affect the number, size, and distribution of …

A transcription factor-based biosensor for detection of itaconic acid

EKR Hanko, NP Minton, N Malys - ACS synthetic biology, 2018 - ACS Publications
Itaconic acid is an important platform chemical that can easily be incorporated into polymers
and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number …

Identification of the haloarchaeal phasin (PhaP) that functions in polyhydroxyalkanoate accumulation and granule formation in Haloferax mediterranei

S Cai, L Cai, H Liu, X Liu, J Han, J Zhou… - Applied and …, 2012 - Am Soc Microbiol
The polyhydroxyalkanoate (PHA) granule-associated proteins (PGAPs) are important for
PHA synthesis and granule formation, but currently little is known about the haloarchaeal …

Identification of a multifunctional protein, PhaM, that determines number, surface to volume ratio, subcellular localization and distribution to daughter cells of poly (3 …

D Pfeiffer, A Wahl, D Jendrossek - Molecular microbiology, 2011 - Wiley Online Library
A two‐hybrid approach was applied to screen for proteins with the ability to interact with PHB
synthase (PhaC1) of Ralstonia eutropha. The H16_A0141 gene (phaM) was identified in the …

Functional genetic elements for controlling gene expression in Cupriavidus necator H16

S Alagesan, EKR Hanko, N Malys… - Applied and …, 2018 - Am Soc Microbiol
ABSTRACT A robust and predictable control of gene expression plays an important role in
synthetic biology and biotechnology applications. Development and quantitative evaluation …

PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha

A Wahl, N Schuth, D Pfeiffer, S Nussberger… - BMC microbiology, 2012 - Springer
Abstract Background Poly (3-hydroxybutyrate)(PHB) granules are important storage
compounds of carbon and energy in many prokaryotes which allow survival of the cells in …