[PDF][PDF] A simple guide to de novo transcriptome assembly and annotation

V Raghavan, L Kraft, F Mesny… - Briefings in …, 2022 - academic.oup.com
A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

Convergent evolution of plant prickles by repeated gene co-option over deep time

JW Satterlee, D Alonso, P Gramazio, KM Jenike, J He… - Science, 2024 - science.org
An enduring question in evolutionary biology concerns the degree to which episodes of
convergent trait evolution depend on the same genetic programs, particularly over long …

A reference genome sequence for giant sequoia

AD Scott, AV Zimin, D Puiu, R Workman… - G3: Genes …, 2020 - academic.oup.com
The giant sequoia (Sequoiadendron giganteum) of California are massive, long-lived trees
that grow along the US Sierra Nevada mountains. Genomic data are limited in giant sequoia …

High-quality chromosome-scale assembly of the walnut (Juglans regia L.) reference genome

A Marrano, M Britton, PA Zaini, AV Zimin… - …, 2020 - academic.oup.com
Background The release of the first reference genome of walnut (Juglans regia L.) enabled
many achievements in the characterization of walnut genetic and functional variation …

Assembled and annotated 26.5 Gbp coast redwood genome: a resource for estimating evolutionary adaptive potential and investigating hexaploid origin

DB Neale, AV Zimin, S Zaman, AD Scott, B Shrestha… - G3, 2022 - academic.oup.com
Sequencing, assembly, and annotation of the 26.5 Gbp hexaploid genome of coast redwood
(Sequoia sempervirens) was completed leading toward discovery of genes related to …

Genomic signatures of disease resistance in endangered staghorn corals

SV Vollmer, JD Selwyn, BA Despard, CL Roesel - Science, 2023 - science.org
White band disease (WBD) has caused unprecedented declines in the Caribbean Acropora
corals, which are now listed as critically endangered species. Highly disease-resistant …

Somatic mutation rates scale with time not growth rate in long-lived tropical trees

A Satake, R Imai, T Fujino, S Tomimoto, K Ohta… - Elife, 2024 - elifesciences.org
The rates of appearance of new mutations play a central role in evolution. However,
mutational processes in natural environments and their relationship with growth rates are …

Reference-free assembly of long-read transcriptome sequencing data with RNA-Bloom2

KM Nip, S Hafezqorani, KK Gagalova, R Chiu… - Nature …, 2023 - nature.com
Long-read sequencing technologies have improved significantly since their emergence.
Their read lengths, potentially spanning entire transcripts, is advantageous for …

Co-option of wing-patterning genes underlies the evolution of the treehopper helmet

CR Fisher, JL Wegrzyn, EL Jockusch - Nature Ecology & Evolution, 2020 - nature.com
Understanding the origin of novelty is a key question in evolutionary developmental biology.
In arthropods, the body wall has served as a repeated source of morphological novelty. In …

Autoactivation of mycorrhizal symbiosis signaling through gibberellin deactivation in orchid seed germination

C Miura, Y Furui, T Yamamoto, Y Kanno… - Plant …, 2024 - academic.oup.com
Orchids parasitically depend on external nutrients from mycorrhizal fungi for seed
germination. Previous findings suggest that orchids utilize a genetic system of mutualistic …