Tuberculosis treatment monitoring and outcome measures: new interest and new strategies

J Heyckendorf, SB Georghiou, N Frahm… - Clinical microbiology …, 2022 - Am Soc Microbiol
Despite the advent of new diagnostics, drugs and regimens, tuberculosis (TB) remains a
global public health threat. A significant challenge for TB control efforts has been the …

Gut mycobiota alterations in patients with COVID-19 and H1N1 infections and their associations with clinical features

L Lv, S Gu, H Jiang, R Yan, Y Chen, Y Chen… - Communications …, 2021 - nature.com
The relationship between gut microbes and COVID-19 or H1N1 infections is not fully
understood. Here, we compared the gut mycobiota of 67 COVID-19 patients, 35 H1N1 …

Unlocking environmental contamination of animal tuberculosis hotspots with viable mycobacteria at the intersection of flow cytometry, PCR, and ecological modelling

AC Pereira, D Pinto, MV Cunha - Science of the Total Environment, 2023 - Elsevier
Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTBC),
circulates in multi-host mammal communities. While interactions between different host …

Quantifying intracellular Mycobacterium tuberculosis: An essential issue for in vitro assays

DC Rodriguez, M Ocampo, LM Salazar… - …, 2018 - Wiley Online Library
Many studies about intracellular microorganisms which are important regarding diseases
affecting public health have been focused on the recognition of host–pathogen interactions …

Differentiation of live and dead Mycobacterium tuberculosis complex in meat samples using PMA qPCR

S Dorn-In, M Gareis, K Schwaiger - Food microbiology, 2019 - Elsevier
The causative agents of zoonotic bovine tuberculosis (bTB), Mycobacterium bovis and M.
caprae, are members of the M. tuberculosis complex (MTC). Wildlife such as red deer …

Subtracting the background by reducing cell-free DNA's confounding effects on Mycobacterium tuberculosis quantitation and the sputum microbiome

CC Naidoo, R Venter, F Codony, G Agustí, N Kitchin… - Scientific Reports, 2024 - nature.com
DNA characterisation in people with tuberculosis (TB) is critical for diagnostic and
microbiome evaluations. However, extracellular DNA, more frequent in people on …

Direct detection from clinical sputum samples to differentiate live and dead Mycobacterium Tuberculosis

J Lu, H Zheng, P Chu, S Han, H Yang… - Journal of Clinical …, 2019 - Wiley Online Library
Background In this study, we aimed to optimize the condition of propidium monoazide (PMA)
treatment for direct detection of Mycobacterium tuberculosis (MTB) from clinical specimens …

[HTML][HTML] Detection and quantification of viable and nonviable Trypanosoma cruzi parasites by a propidium monoazide real-time polymerase chain reaction assay

B Cancino-Faure, R Fisa, MM Alcover… - The American journal …, 2016 - ncbi.nlm.nih.gov
Molecular techniques based on real-time polymerase chain reaction (qPCR) allow the
detection and quantification of DNA but are unable to distinguish between signals from dead …

Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells

XA Kayigire, SO Friedrich, MN Karinja… - Tuberculosis, 2016 - Elsevier
Propidium monoazide (PMA) penetrates non-viable cells with compromised membranes.
PMA has been proposed to improve the specificity of Xpert MTB/RIF (Xpert) for the detection …

International collaboration among medical societies is an effective way to boost Latin American production of articles on tuberculosis

GB Migliori, R Centis, L D'Ambrosio… - Jornal Brasileiro de …, 2019 - SciELO Brasil
Objective: Most studies of tuberculosis originate from high-income countries with a low
incidence of tuberculosis. A review of the scientific production on tuberculosis in Latin …