Histology permits the observation of otherwise invisible structures of the internal topography
of a specimen. Although it enables the investigation of tissues at a cellular level, it is invasive …

The common image related artifacts during image acquisition are noise caused due to
external interference and imbalance in illumination. Uneven illumination correction …

A flat-field correction method is proposed for multiple measured hyperspectral microscopy
imaging in this paper. As the most crucial preprocessing process in quantitative microscopic …

This chapter lays out the elementary principles of fractal geometry underpinning much of the
rest of this book. It assumes a minimal mathematical background, defines the key principles …

Calcium imaging is an essential tool to study the activity of neuronal populations. However,
the high level of background fluorescence in images hinders the accurate identification of …

Significance Multiphoton microscopy (MPM) is a useful biomedical imaging tool for its ability
to probe labeled and unlabeled depth-resolved tissue biomarkers at high resolution …

Spatial transcriptomics techniques such as STARmap [15] enable the subcellular detection
of RNA transcripts within complex tissue sections. The data from these techniques are …

With the recent advances of optical tissue clearing technology, current imaging modalities
are able to image large tissue samples in 3D with single-cell resolution. However, the …

The efficacy of cancer treatments (eg, radiotherapy, chemotherapy, etc.) has been observed
to critically depend on the proportion of hypoxic regions (ie, a region deprived of adequate …

Image quality, resolution and scanning time are critical in digital pathology. In order to create
a high-resolution digital image, the scanner systems execute stitching algorithms to the …