The emerging landscape of spatial profiling technologies
Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein
profiling methods as a major pillar for cellular atlas building of complex samples, from …
profiling methods as a major pillar for cellular atlas building of complex samples, from …
RNA sequencing: the teenage years
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …
transcriptome-wide analysis of differential gene expression and differential splicing of …
DestVI identifies continuums of cell types in spatial transcriptomics data
Most spatial transcriptomics technologies are limited by their resolution, with spot sizes
larger than that of a single cell. Although joint analysis with single-cell RNA sequencing can …
larger than that of a single cell. Although joint analysis with single-cell RNA sequencing can …
Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas
Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell
populations in a tissue, but characterizing their spatial organization remains challenging. We …
populations in a tissue, but characterizing their spatial organization remains challenging. We …
Clinical and translational values of spatial transcriptomics
L Zhang, D Chen, D Song, X Liu, Y Zhang… - … and Targeted Therapy, 2022 - nature.com
The combination of spatial transcriptomics (ST) and single cell RNA sequencing (scRNA-
seq) acts as a pivotal component to bridge the pathological phenomes of human tissues with …
seq) acts as a pivotal component to bridge the pathological phenomes of human tissues with …
[HTML][HTML] A spatiotemporal organ-wide gene expression and cell atlas of the developing human heart
The process of cardiac morphogenesis in humans is incompletely understood. Its full
characterization requires a deep exploration of the organ-wide orchestration of gene …
characterization requires a deep exploration of the organ-wide orchestration of gene …
Spatiotemporal dynamics of molecular pathology in amyotrophic lateral sclerosis
Paralysis occurring in amyotrophic lateral sclerosis (ALS) results from denervation of
skeletal muscle as a consequence of motor neuron degeneration. Interactions between …
skeletal muscle as a consequence of motor neuron degeneration. Interactions between …
Single-cell RNA sequencing in cardiovascular development, disease and medicine
Advances in single-cell RNA sequencing (scRNA-seq) technologies in the past 10 years
have had a transformative effect on biomedical research, enabling the profiling and analysis …
have had a transformative effect on biomedical research, enabling the profiling and analysis …
Alignment and integration of spatial transcriptomics data
Spatial transcriptomics (ST) measures mRNA expression across thousands of spots from a
tissue slice while recording the two-dimensional (2D) coordinates of each spot. We …
tissue slice while recording the two-dimensional (2D) coordinates of each spot. We …
Integrative methods and practical challenges for single-cell multi-omics
Fast-developing single-cell multimodal omics (scMulti-omics) technologies enable the
measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA …
measurement of multiple modalities, such as DNA methylation, chromatin accessibility, RNA …