BRAQUE: Bayesian reduction for amplified quantization in UMAP embedding

L Dall'Olio, M Bolognesi, S Borghesi, G Cattoretti… - Entropy, 2023 - mdpi.com
Single-cell biology has revolutionized the way we understand biological processes. In this
paper, we provide a more tailored approach to clustering and analyzing spatial single-cell …

Validation of an Organ Mapping Antibody Panel for Cyclical Immunofluorescence Microscopy on Normal Human Kidneys

M Brewer, LG Migas, KA Clouthier… - American Journal …, 2024 - journals.physiology.org
The lack of standardization in antibody validation remains a major contributor to
irreproducibility of human research. To address this, we have applied a standardized …

Rejuvenated vintage tissue sections highlight individual antigen fate during processing and long-term storage

F Mascadri, MM Bolognesi, D Pilla… - … of Histochemistry & …, 2021 - journals.sagepub.com
Antigen-bearing proteins become progressively unavailable to immunodetection after
prolonged storage of routine sections, exposed to a variety of agents, such as moisture …

A DNA damage response‐like phenotype defines a third of colon cancers at onset

S Mauro, MM Bolognesi, N Villa, G Capitoli… - The FASEB …, 2023 - Wiley Online Library
Colon adenocarcinoma (COAD) has a limited range of diversified, personalized therapeutic
opportunities, besides DNA hypermutating cases; thus, both new targets or broadening …

Seeing or believing in hyperplexed spatial proteomics via antibodies: New and old biases for an image-based technology

MM Bolognesi, L Dall'Olio, A Maerten, S Borghesi… - Biological …, 2024 - cambridge.org
Hyperplexed in-situ targeted proteomics via antibody immunodetection (ie,> 15 markers) is
changing how we classify cells and tissues. Differently from other high-dimensional single …

A DNA damage response-like phenotype defines a novel subset of colon cancer

S Mauro, M Bolognesi, N Villa, G Capitoli, L Furia… - 2022 - researchsquare.com
Colon adenocarcinoma (COAD) has a limited range of diversified, personalized therapeutic
opportunities, besides DNA hypermutating cases; thus both new targets or broadening …