The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Abstract The RNA polymerase II (Pol II) pre-initiation complex (PIC) is a critical node in
eukaryotic transcription regulation, and its formation is the major rate-limiting step in …

Alterations in the regulation of gene expression are frequently associated with
developmental diseases or cancer. Transcription activation is a key phenomenon in the …

Transcription of eukaryotic protein-coding genes commences with the assembly of a
conserved initiation complex, which consists of RNA polymerase II (Pol II) and the general …

The conserved co-activator complex Mediator enables regulated transcription initiation by
RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) …

The Mediator complex is a multi-subunit assembly that appears to be required for regulating
expression of most RNA polymerase II (pol II) transcripts, which include protein-coding and …

▪ Abstract The events leading to transcription of eukaryotic protein-coding genes culminate
in the positioning of RNA polymerase II at the correct initiation site. The core promoter, which …

In eukaryotes, the core promoter serves as a platform for the assembly of transcription
preinitiation complex (PIC) that includes TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, and RNA …

Here we review recent advances in understanding the regulation of mRNA synthesis in
Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been …

Faithful segregation of replicated chromosomes is essential for maintenance of genetic
stability and seems to be monitored by several mitotic checkpoints. Various components of …