A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification
Extremophilic proteins are valuable in various fields, but their expression can be challenging
in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax …
in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax …
Cloning and expression of recombinant human superoxide dismutase 1 (hSOD1) in Bacillus subtilis 1012
M Yin, N Wang, Q Wang, H Xia, X Cheng, H Hu… - Biotechnology …, 2023 - Springer
Objective We aimed to clone and express the human Cu, Zn superoxide dismutase (hSOD1)
in Bacillus subtilis 1012. Also, we investigated the expression level of hSOD1 under different …
in Bacillus subtilis 1012. Also, we investigated the expression level of hSOD1 under different …
Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome Editing
S Agrawal, MH Padmaswari, AL Stokes… - Biomedicines, 2024 - mdpi.com
The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering
unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from …
unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from …
Development of an immunodiagnostic assay for the detection of Sugarcane mosaic virus
HM Khalid, NUSS SADAF ZAIDI… - Turkish Journal of …, 2024 - journals.tubitak.gov.tr
Background/Aim: Sugarcane mosaic virus (SCMV; genus Potyvirus and family Potyviridae),
poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim …
poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim …
N‐terminal LysSN‐His‐tag improves the production of intracellular recombinant protein in Bacillus subtilis
NTP Le, TTP Phan, TTT Truong… - Cell Biochemistry …, 2023 - Wiley Online Library
Choosing fusion tags to enhance the recombinant protein levels in the cytoplasm of Bacillus
subtilis has been limited. Our previous study demonstrated that His‐tag at the N‐terminus …
subtilis has been limited. Our previous study demonstrated that His‐tag at the N‐terminus …
A novel epitope tag from rabies virus has versatile in vitro applications
Y Zhang, L Zhao, Q Wang, Z Zhang, X Ren… - Applied Microbiology …, 2023 - Springer
Fusion tag technology is an important tool for rapid separation, purification, and
characterization of proteins. Combined with monoclonal antibodies, tag epitope systems can …
characterization of proteins. Combined with monoclonal antibodies, tag epitope systems can …