The interplay between replication stress and the S phase checkpoint is a key determinant of
genome maintenance, and has a major impact on human diseases, notably, tumour …

DNA replication results in the doubling of the genome prior to cell division. This process
requires the assembly of 50 or more protein factors into a replication fork. Here, we review …

Accurate replication of DNA requires stringent regulation to ensure genome integrity. In
human cells, thousands of origins of replication are coordinately activated during S phase …

Minichromosome maintenance proteins (MCMs) are DNA-dependent ATPases that bind to
replication origins and license them to support a single round of DNA replication. A large …

Excess dormant origins bound by the minichromosome maintenance (MCM) replicative
helicase complex play a critical role in preventing replication stress, chromosome instability …

Eukaryotes use distinct polymerases for leading-and lagging-strand replication, but how
they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae …

Loading of the eukaryotic replicative helicase onto replication origins involves two MCM
hexamers forming a double hexamer (DH) around duplex DNA. During S phase, helicase …

Human development and tissue homeostasis depend on the regulated control of cellular
proliferation and differentiation. DNA replication is essential to couple genome duplication …

ATR kinase activity slows replication forks and prevents origin firing in damaged cells. Here
we describe proteomic analyses that identified mechanisms through which ATR kinase …

Recently published structural and functional analyses of the CMG complex have provided
insight into the mechanism of its DNA helicase function and into the distinct roles of its …