Exercise and the gut microbiome: a review of the evidence, potential mechanisms, and implications for human health

LJ Mailing, JM Allen, TW Buford… - Exercise and sport …, 2019 - journals.lww.com
The gastrointestinal tract contains trillions of microbes (collectively known as the gut
microbiota) that play essential roles in host physiology and health. Studies from our group …

Reintroducing mothur: 10 years later

PD Schloss - Applied and environmental microbiology, 2020 - Am Soc Microbiol
More than 10 years ago, we published the paper describing the mothur software package in
Applied and Environmental Microbiology. Our goal was to create a comprehensive package …

Consistent and correctable bias in metagenomic sequencing experiments

MR McLaren, AD Willis, BJ Callahan - Elife, 2019 - elifesciences.org
Marker-gene and metagenomic sequencing have profoundly expanded our ability to
measure biological communities. But the measurements they provide differ from the truth …

Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies

DM Gohl, P Vangay, J Garbe, A MacLean… - Nature …, 2016 - nature.com
Amplicon-based marker gene surveys form the basis of most microbiome and other
microbial community studies. Such PCR-based methods have multiple steps, each of which …

Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?

E Ringø, Z Zhou, JLG Vecino, S Wadsworth… - Aquaculture …, 2016 - Wiley Online Library
It is well known that healthy gut microbiota is essential to promote host health and well‐
being. The intestinal microbiota of endothermic animals as well as fish are classified as …

Understanding PCR processes to draw meaningful conclusions from environmental DNA studies

RP Kelly, AO Shelton, R Gallego - Scientific reports, 2019 - nature.com
As environmental DNA (eDNA) studies have grown in popularity for use in ecological
applications, it has become clear that their results differ in significant ways from those of …

Single-cell analysis tools for drug discovery and development

JR Heath, A Ribas, PS Mischel - Nature reviews Drug discovery, 2016 - nature.com
The genetic, functional or compositional heterogeneity of healthy and diseased tissues
presents major challenges in drug discovery and development. Such heterogeneity hinders …

Primer and platform effects on 16S rRNA tag sequencing

J Tremblay, K Singh, A Fern, ES Kirton, S He… - Frontiers in …, 2015 - frontiersin.org
Sequencing of 16S rRNA gene tags is a popular method for profiling and comparing
microbial communities. The protocols and methods used, however, vary considerably with …

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

KR Theis, R Romero, AD Winters, JM Greenberg… - American journal of …, 2019 - Elsevier
Background The human placenta has been traditionally viewed as sterile, and microbial
invasion of this organ has been associated with adverse pregnancy outcomes. Yet, recent …

Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis

TM Porter, M Hajibabaei - Molecular ecology, 2018 - Wiley Online Library
The purpose of this review is to present the most common and emerging DNA‐based
methods used to generate data for biodiversity and biomonitoring studies. As environmental …