Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Prospects of surface-enhanced Raman spectroscopy for biomarker monitoring toward precision medicine
Future precision medicine will be undoubtedly sustained by the detection of validated
biomarkers that enable a precise classification of patients based on their predicted disease …
biomarkers that enable a precise classification of patients based on their predicted disease …
Classification of T-cell activation via autofluorescence lifetime imaging
AJ Walsh, KP Mueller, K Tweed, I Jones… - Nature biomedical …, 2021 - nature.com
The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …
KRAS controls pancreatic cancer cell lipid metabolism and invasive potential through the lipase HSL
CN Rozeveld, KM Johnson, L Zhang, GL Razidlo - Cancer research, 2020 - AACR
Oncogene-induced metabolic reprogramming is a hallmark of pancreatic cancer (PDAC), yet
the metabolic drivers of metastasis are unclear. In PDAC, obesity and excess fatty acids …
the metabolic drivers of metastasis are unclear. In PDAC, obesity and excess fatty acids …
Deep and dynamic metabolic and structural imaging in living tissues
Label-free imaging through two-photon autofluorescence of NAD (P) H allows for
nondestructive, high-resolution visualization of cellular activities in living systems. However …
nondestructive, high-resolution visualization of cellular activities in living systems. However …
Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis
E Sánchez-Ramírez, TPL Ung… - Journal of Cell …, 2022 - rupress.org
Adipocytes are the main cell type in adipose tissue, which is a critical regulator of
metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from …
metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from …
Fluorescence Lifetime Imaging Microscopy (FLIM) reveals spatial-metabolic changes in 3D breast cancer spheroids
Cancer cells are mechanically sensitive to physical properties of the microenvironment,
which can affect downstream signaling to promote malignancy, in part through the …
which can affect downstream signaling to promote malignancy, in part through the …
[HTML][HTML] Multimodal analyses of vitiligo skin idenitfy tissue characteristics of stable disease
Vitiligo is an autoimmune skin disease characterized by the destruction of melanocytes by
autoreactive CD8+ T cells. Melanocyte destruction in active vitiligo is mediated by CD8+ T …
autoreactive CD8+ T cells. Melanocyte destruction in active vitiligo is mediated by CD8+ T …
3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors
D Sood, M Tang-Schomer, D Pouli, C Mizzoni… - Nature …, 2019 - nature.com
Dynamic alterations in the unique brain extracellular matrix (ECM) are involved in malignant
brain tumors. Yet studies of brain ECM roles in tumor cell behavior have been difficult due to …
brain tumors. Yet studies of brain ECM roles in tumor cell behavior have been difficult due to …
A mitochondrial NADPH-cholesterol axis regulates extracellular vesicle biogenesis to support hematopoietic stem cell fate
Mitochondrial fatty acid oxidation (FAO) is essential for hematopoietic stem cell (HSC) self-
renewal; however, the mechanism by which mitochondrial metabolism controls HSC fate …
renewal; however, the mechanism by which mitochondrial metabolism controls HSC fate …