It is now firmly established that the contraction of muscle and many of the movements of
nonmuscle cells result from the interaction of two proteins, actin and myosin. These …

This review deals with the regulatory system of striated muscle. In current parlance, the term
regulatory system refers to those proteins that are located in the so-called thin filaments of …

Fluorescence spectroscopy and its applications to the physical and life sciences have
evolved rapidly during the past decade. The increased interest in fluorescence appears to …

Knowledge of the elastic properties of actin filaments is crucial for considering its role in
muscle contraction, cellular motile events, and formation of cell shape. The stiffness of actin …

Microtubule-associated proteins regulate microtubule dynamics, bundle actin filaments, and
cross-link actin filaments with microtubules. In addition, aberrant interaction of the …

Cofilin is essential for cell viability and for actin-based motility. Cofilin severs actin filaments,
which enhances the dynamics of filament assembly. We investigated the mechanism of …

To test the hypothesis that the myosin II motor domain (S1) preferentially binds to specific
subsets of actin filaments in vivo, we expressed GFP-fused S1 with mutations that enhanced …

We have measured the effects of cofilin on the conformation and dynamics of actin filaments
labeled at Cys374 with erythrosin-iodoacetemide (ErIA), using time-resolved …

Fluorescence anisotropy decay belongs to the general class of relaxation methods which
monitor the time dependence of the transition of the system from a biased to a random …

A new approach is presented for measuring the three-dimensional orientation of individual
macromolecules using single molecule fluorescence polarization (SMFP) microscopy. The …