Transcription-factor-based biosensors (TFBs) are often used for metabolite detection,
adaptive evolution, and metabolic flux control. However, designing TFBs with superior …

In this review, we address recent advances made in pathway engineering, directed
evolution, and systems/synthetic biology approaches employed in the production and …

Global energy demand and environmental concerns have stimulated increasing efforts to
produce carbon-neutral fuels directly from renewable resources. Microbially derived …

Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …

Due to the ability to regulate target metabolic pathways globally and dynamically, metabolic
regulation systems composed of transcription factors have been widely used in metabolic …

Plants possess myriads of secondary metabolites with a broad spectrum of health-promoting
benefits. To date, plant extraction is still the primary route to produce high-value natural …

Living organisms have evolved a plethora of sensing systems for the intra-and extracellular
detection of small molecules, ions or physical parameters. Several recent studies have …

Metabolite biosensors are central to current efforts toward precision engineering of
metabolism. Although most research has focused on building new biosensors, their …

Quantification and regulation of pathway metabolites is crucial for optimization of microbial
production bioprocesses. Genetically encoded biosensors provide the means to couple …

Highlights•Synthetic biology provides new tools for the dynamic control of
metabolism.•Dynamic control is crucial to further the field of metabolic engineering.•Sensors …