There is a lack of new antibiotics to combat drug-resistant bacterial infections that
increasingly threaten global health. The current pipeline of clinical-stage antimicrobials is …

The prevalence of antimicrobial resistance in Gram-negative bacteria poses a greater
challenge due to their intrinsic resistance to many antibiotics. Recently, darobactins have …

The outer membrane is a formidable barrier that protects Gram-negative bacteria against
environmental threats. Its integrity requires the correct folding and insertion of outer …

The t ranslocation and a ssembly m odule (TAM) has been proposed to play a crucial role in
the assembly of a small subset of outer membrane proteins (OMPs) in Proteobacteria based …

BamA, the core component of the β-barrel assembly machinery (BAM) complex, is an outer-
membrane protein (OMP) in Gram-negative bacteria. Its function is to insert and fold …

Biosynthetic engineering of bicyclic darobactins, selectively sealing the lateral gate of the
outer membrane protein BamA, leads to active analogues, which are up to 128-fold more …

In-cell electron paramagnetic resonance (EPR) spectroscopy experiments provide high-
resolution data about conformational changes of proteins within the cell. However, one of …

The study of how the intracellular medium influences protein structural dynamics and protein–
protein interactions is a captivating area of research for scientists aiming to comprehend …

The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs)
from aggregation in the periplasm of Gram-negative bacteria, and delivers them to the β …

The Gram-negative selective antibiotic darobactin A has attracted interest owing to its
intriguing fused bicyclic structure and unique targeting of the outer membrane protein BamA …