A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast
M Rodríguez-López, C Cotobal… - Wellcome open …, 2017 - pmc.ncbi.nlm.nih.gov
In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene
manipulations is based on homologous recombination of a PCR product that contains …
manipulations is based on homologous recombination of a PCR product that contains …
[HTML][HTML] RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly
In eukaryotes, the Suv39 family of proteins tri-methylate lysine 9 of histone H3 (H3K9me) to
form constitutive heterochromatin. However, how Suv39 proteins are nucleated at …
form constitutive heterochromatin. However, how Suv39 proteins are nucleated at …
GetPrimers: A generalized PCR‐based genetic targeting primer designer enabling easy and standardized targeted gene modification across multiple systems
Z Miao, H Wang, X Tu, Z Huang, S Huang, X Zhang… - Yeast, 2024 - Wiley Online Library
Genetic targeting (eg, gene knockout and tagging) based on polymerase chain reaction
(PCR) is a simple yet powerful approach for studying gene functions. Although originally …
(PCR) is a simple yet powerful approach for studying gene functions. Although originally …
The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability
The target of Rapamycin complex1 (TORC1) senses and integrates several environmental
signals, including amino acid (AA) availability, to regulate cell growth. Folliculin (FLCN) is a …
signals, including amino acid (AA) availability, to regulate cell growth. Folliculin (FLCN) is a …
Yesprit and Yeaseq: Applications for designing primers and browsing sequences for research using the four Schizosaccharomyces species
X Wang, R Xu, Y Wang, Z Liu, R Lou, T Sugiyama - Yeast, 2021 - Wiley Online Library
The polymerase chain reaction (PCR)‐based gene targeting method, which can delete a
specific gene or introduce tags, has been widely utilized to study gene function in fission …
specific gene or introduce tags, has been widely utilized to study gene function in fission …
[PDF][PDF] A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast [version 3; referees: 2 approved]
M Rodríguez-López, C Cotobal… - Wellcome Open …, 2017 - pmc.ncbi.nlm.nih.gov
In the fission yeast the prevailing approach for Schizosaccharomyces pombe gene
manipulations is based on homologous recombination of a PCR product that contains …
manipulations is based on homologous recombination of a PCR product that contains …