The use of predictive models to develop chromatography-based purification processes

CR Bernau, M Knödler, J Emonts, RC Jäpel… - … in Bioengineering and …, 2022 - frontiersin.org
Chromatography is the workhorse of biopharmaceutical downstream processing because it
can selectively enrich a target product while removing impurities from complex feed streams …

Glucose and Maltose Surface-Functionalized Thermoresponsive Poly(N-Vinylcaprolactam) Nanogels

J Siirila, S Hietala, FS Ekholm, H Tenhu - Biomacromolecules, 2020 - ACS Publications
Soft nanoparticles are interesting materials due to their size, deformability, and ability to host
guest molecules. Surface properties play an essential role in determining the fate of the …

Controlling external versus internal pore modification of ultrafiltration membranes using surface-initiated AGET-ATRP

BM Carter, A Sengupta, X Qian, M Ulbricht… - Journal of Membrane …, 2018 - Elsevier
The activators generated electron transfer (AGET) method coupled with atom transfer radical
polymerization (ATRP) is shown to be a simple and well controlled approach for surface …

How environmental factors affect the structural properties and biofunctions of keratin: a molecular dynamics study

C Yin, L Feng, N Zhang, Y Cheng - Materials Today Communications, 2023 - Elsevier
Keratin is the primary component of stratum corneum (SC). Due to the abundant resources
and intrinsic biophysical properties, such as skin, hair, horns, wools, hooves and so on …

Atomistic Molecular Dynamics Simulations of the Lower Critical Solution Temperature Transition of Poly(N-vinylcaprolactam) in Aqueous Solutions

X Sun, X Qian - The Journal of Physical Chemistry B, 2019 - ACS Publications
Poly (N-vinylcaprolactam)(PVCL) is a thermo-responsive polymer, which exhibits a lower
critical solution temperature (LCST) in an aqueous solution. The LCST of this hydrophilic-to …

Electrospun weak anion-exchange fibrous membranes for protein purification

ST Chen, SR Wickramasinghe, X Qian - Membranes, 2020 - mdpi.com
Membrane based ion-exchange (IEX) and hydrophobic interaction chromatography (HIC) for
protein purification is often used to remove impurities and aggregates operated under the …

Multifunctionalization of RC membrane via combining surface initiated RAFT polymerization with thiolactone chemistry for enhanced antibody recovery

J Ye, M Guo, C Han, Y Zhang, J Meng - Polymer, 2022 - Elsevier
Hydrophobic charge induction chromatography (HCIC) is becoming an attractive separation
technology for antibody purification but suffers from low recovery. Herein we attempted to …

Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification

ST Chen, SR Wickramasinghe, X Qian - Membranes, 2022 - mdpi.com
Responsive membranes for hydrophobic interaction chromatography have been fabricated
by functionalizing poly (N-vinylcaprolactam)(PVCL) ligands on the substrate of electrospun …

Molecular dynamics of cobalt protoporphyrin antagonism of the cancer suppressor REV-ERBβ

TM Fakih, F Kurniawan, M Yusuf, M Mudasir… - Molecules, 2021 - mdpi.com
Nuclear receptor REV-ERBβ is an overexpressed oncoprotein that has been used as a
target for cancer treatment. The metal-complex nature of its ligand, iron protoporphyrin IX …

Aggregate Removal by Responsive Electrospun Membrane based Hydrophobic Interaction Chromatography

X Hao, ST Chen, YH Chiao… - Chemie Ingenieur …, 2024 - Wiley Online Library
Abstract Poly (N‐vinylcaprolactam)(PVCL), a salt responsive ligand, was grafted from
electrospun cellulose nanofiber membranes by atom transfer radical polymerization (ATRP) …