miRNAs can be found in serum and other body fluids and serve as biomarkers for disease.
More importantly, secreted miRNAs, especially those in extracellular vesicles (EVs) such as …

Next-generation sequencing technologies have revolutionarily advanced sequence-based
research with the advantages of high-throughput, high-sensitivity, and high-speed. RNA-seq …

Background Gene set scoring provides a useful approach for quantifying concordance
between sample transcriptomes and selected molecular signatures. Most methods use …

As the use of RNA-seq has popularized, there is an increasing consciousness of the
importance of experimental design, bias removal, accurate quantification and control of false …

To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both
RNA-Seq and microarray analyses were performed on RNA samples from a human T cell …

We introduce Sailfish, a computational method for quantifying the abundance of previously
annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping …

The acceleration of DNA sequencing in samples from patients and population studies has
resulted in extensive catalogues of human genetic variation, but the interpretation of rare …

Abstract Background Transcriptome sequencing (RNA-Seq) has become the assay of
choice for high-throughput studies of gene expression. However, as is the case with …

The transcriptomics field has developed rapidly with the advent of next-generation
sequencing technologies. RNA-seq has now displaced microarrays as the preferred method …

Our understanding of gene expression has changed dramatically over the past decade,
largely catalysed by technological developments. High-throughput experiments …