Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Recombinant protein production emerged in the early 1980s with the development of
genetic engineering tools, which represented a compelling alternative to protein extraction …

Ferredoxins comprise a large family of iron–sulfur (Fe–S) proteins that shuttle electrons in
diverse biological processes. Human mitochondria contain two isoforms of [2Fe-2S] …

The optimization of engineered metabolic pathways requires careful control over the levels
and timing of metabolic enzyme expression,,,. Optogenetic tools are ideal for achieving such …

RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein
aggregates or through phase separation into a liquid RNA/protein phase. Which model …

The unfolded protein response (UPR) is a conserved homeostatic program that is activated
by misfolded proteins in the lumen of the endoplasmic reticulum (ER). Recently, it became …

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently
been developed. However, especially when multiple genetic manipulations are required …

CRISPR/Cas9 is a simple and efficient tool for targeted and marker-free genome
engineering. Here, we report the development and successful application of a multiplex …

Ageing results from complex genetically and epigenetically programmed processes that are
elicited in part by noxious or stressful events that cause programmed cell death. Here, we …

Despite the extensive use of Saccharomyces cerevisiae as a platform for synthetic biology,
strain engineering remains slow and laborious. Here, we employ CRISPR/Cas9 technology …