The hammerhead ribozyme is a small RNA motif that self cleaves at a specific
phosphodiester bond to produce 2′, 3′ cyclic phosphate and 5′ hydroxyl termini …

The emerging knowledge about RNA-based enzymes has already had great impact on our
concept of evolutionary history, making the 'RNA world'more likely. It may well have an …

Evidence for a two-metal ion mechanism for cleavage of the HH16 hammerhead ribozyme is
provided by monitoring the rate of cleavage of the RNA substrate as a function of La3+ …

Significant cleavage by hammerhead ribozymes requires activation by divalent metal ions.
Several models have been proposed to account for the influence of metal ions on …

The NMR solution structure of a lead-dependent ribozyme, known as the leadzyme, is
presented. This ribozyme is among the smallest of the known catalytic RNAs, with an active …

A site-specific chemical modification strategy has been employed to elucidate structure−
function relationships at the only phylogenetically nonconserved position within the core of …

Leadzyme is a ribozyme that requires Pb2+. We have previously shown that the addition of
Nd3+ in the presence of Pb2+ increased significantly the yield of the RNA cleavage reaction …

The contribution of several individual ribozyme⋅ substrate base pairs to binding and
catalysis has been investigated using hammerhead ribozyme substrates that were truncated …

The capacity of DNA to bind RNA via Watson-Crick base-pairing is fundamental to antisense
oligonucleotide strategies to inhibit gene expression, and is a property that has been …

The CRISPR‐Cas systems are adopted as powerful molecular tools for not only genetic
manipulation but also point‐of‐care diagnostics. However, methods to enable diagnostics of …